Rat CXCL1/GRO alpha /KC/CINC-1 Antibody

Catalog #: MAB5151 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB5151-100
MAB5151-SP

Detection of Rat CXCL1/GRO alpha /KC/CINC‑1 by Western Blot.

2 Images

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat CXCL1/GRO alpha /KC/CINC-1 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CXCL1/GRO alpha /KC/CINC‑1 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2176C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant rat CXCL1/GRO alpha /KC/CINC‑1
Ala25-Lys96
Accession # P14095

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Neutralization

Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.075-0.45 µg/mL in the presence of 4 ng/mL Recombinant Rat CXCL1/GRO alpha /KC/CINC‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Rat CXCL1/GRO alpha /KC/CINC‑1 by Western Blot. Western blot shows lysates of NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Rat CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB5151) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CXCL1/GROa/KC/CINC-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization

View Larger

Chemotaxis Induced by CXCL1/CINC-1 and Neutral-ization by Rat CXCL1/ CINC-1 Antibody. Recombinant Rat CXCL1/CINC-1 (Catalog # 515-CN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL1/CINC-1 (4 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Rat CXCL1/CINC-1 Monoclonal Antibody (Catalog # MAB5151). The ND₅₀ is typically 0.075-45 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL1/GRO alpha/KC/CINC-1

Cytokine-induced Neutrophil Chemoattractant 1 (CINC-1) was originally purified from media conditioned by IL-1 beta stimulated rat kidney epithelioid cells (NRK-52E). On the basis of its protein and DNA sequences, CINC-1 is a member of the alpha (CXC) subfamily of chemokines, designated CXCL1. Three additional rat CXC chemokines (CINC-2 alpha, CINC-2 beta, CINC-3/MIP-2), sharing approximately 63-67% amino acid sequence identity with CINC-1, have been identified. The protein sequence of rat CINC-1 is also 68%, 71%, and 69% identical to that of human GRO-alpha, GRO-beta, and GRO-gamma, respectively. Based on their sequence homology, it has been suggested that CINCs are the rat counterpart of human GROs. CINC-1 is also the counterpart of mouse KC. Rat CINC-1 cDNA encodes a 96 amino acid residue precursor protein from which the amino-terminal 24 amino acid residues are cleaved to generate the mature CINC-1. Similar to other alpha chemokines, rat CINCs are potent neutrophil attractants and activators and have been shown to play an important role in the infiltration of neutrophils into inflammatory sites in rats.

Entrez Gene IDs

2919 (Human); 14825 (Mouse); 81503 (Rat)

Alternate Names

CINC1; CINC-1; CXCL1; FSP; GRO alpha; GRO1; GROa; KC; MGSA; MGSA-a; MGSA-alpha; NAP-3; SCYB1