Rat CXCL3/CINC-2 alpha / beta Biotinylated Antibody

Catalog #: BAF517 Datasheet / COA / SDS

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BAF517

All CXCL3/GRO gamma/CINC-2/DCIP-1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat CXCL3/CINC-2 alpha / beta Biotinylated Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CXCL3/CINC-2 alpha / beta in Western blots. In Western blots, approximately 100% cross‑reactivity with recombinant rat (rr) CINC-2 alpha is observed, 50% cross-reactivity with recombinant mouse (rm) MIP-2 (non-reducing conditions) is observed, 25% cross‑reactivity with rrCINC-1 (non-reducing conditions) is observed, and 5% cross-reactivity with rmKC and recombinant human GRO beta (non-reducing conditions) is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant rat CXCL3/CINC-2 alpha / beta
Arg33-Leu100
Accession # NP_612531

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Rat CXCL3/CINC-2 alpha (Catalog # 516-CA)
Recombinant Rat CXCL3/CINC-2 beta (Catalog # 517-CB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL3/GRO gamma/CINC-2/DCIP-1

CINC-2 alpha and -2 beta are mRNA splice variant isoforms that differ by only 3 amino acids at their amino-termini. These proteins are CXC chemokines most closely related to human GROs and mouse MIP-2. They are produced by granulation tissue, activated macrophages and fibroblasts. CINC-2 biological activities are mediated by CXCR1 or CXCR2.

Entrez Gene IDs

2921 (Human); 330122 (Mouse); 171551 (Rat)

Alternate Names

chemokine (C-X-C motif) ligand 3; CINC-2; CINC-2b; C-X-C motif chemokine 3; CXCL3; Dcip1; Gm1960; GRO gamma; GRO3 oncogene; GRO3; GROG; GRO-gamma; GRO-gamma(1-73); Growth-regulated protein gamma; Macrophage inflammatory protein 2-beta; melanoma growth stimulatory activity gamma; MGSA gamma; MIP2B; MIP-2b; MIP2-beta; SCYB3