Rat CXCR6 Antibody

Catalog #: MAB8196R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8196R-100
MAB8196R-SP

Detection of CXCR6 in HEK293 Human Cell Line Transfected with Rat CXCR6 and eGFP by Flow Cytometry.

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All CXCR6 Antibodies

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Rat CXCR6 Antibody Summary

Species Reactivity

Rat

Specificity

Detects human CXCR6 in ELISAs.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 879112R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with rat CXCR6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CXCR6 in HEK293 Human Cell Line Transfected with Rat CXCR6 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with rat CXCR6 and eGFP was stained with either (A) Mouse Anti-Rat CXCR6 Monoclonal Antibody (Catalog # MAB8196R) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR6

CXCR6, also known as BONZO, STRL33, and CD186, is an approximately 45 kDa 7-TM domain chemokine receptor for the membrane-bound and soluble forms of CXCL16. CXCR6 is expressed on monocytes as well as on NKT, NK, CD4⁺, and CD8⁺ T cells. It is up-regulated in a variety of cancers. CXCR6 mediates the recruitment of immune cells to sites of inflammation and tissue damage. It also promotes NK cell memory and tolerance to NKT cell-mediated graft rejection. CXCR6 enhances the invasiveness of both tumor cells and glial precursor cells. In addition, it can function as a cellular receptor for select variants of HIV types 1 and 2. Rat CXCR6 shares 72% and 88% amino acid sequence identity with human and mouse CXCR6, respectively.

Entrez Gene IDs

10663 (Human); 80901 (Mouse); 100124593 (Rat)

Alternate Names

Bonzo; BONZOG-protein coupled receptor bonzo; CD186 antigen; CD186; CDw186; chemokine (C-X-C motif) receptor 6; C-X-C chemokine receptor type 6; CXCR6; CXC-R6; CXCR-6; G protein-coupled receptor; STRL33; STRL33G-protein coupled receptor STRL33; TYMSTRCD186