Rat CXCR6 PE-conjugated Antibody

Name: Rat CXCR6 PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB8196P

Catalog #: FAB8196P Datasheet / COA / SDS

Discontinued Product

FAB8196P has been discontinued.

View all CXCR6 products.

Detection of CXCR6 in HEK293 Human Cell Line Transfected with Rat CXCR6 and eGFP by Flow Cytometry.

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Rat CXCR6 PE-conjugated Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CXCR6 in ELISA. Stains HEK293 cells transfected with rat CXCR6 by Flow Cytometry, but does not stain irrelevant transfectants.

Source

Monoclonal Mouse IgG₁ Clone # 879112

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with rat CXCR6

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CXCR6 in HEK293 Human Cell Line Transfected with Rat CXCR6 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with rat CXCR6 and eGFP were stained with and either (A) Mouse Anti-Rat CXCR6 PE-conjugated Monoclonal Antibody (Catalog # FAB8196P) or (B) Mouse IgG₁Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR6

CXCR6, also known as BONZO, STRL33, and CD186, is an approximately 45 kDa 7-TM domain chemokine receptor for the membrane-bound and soluble forms of CXCL16. CXCR6 is expressed on monocytes as well as on NKT, NK, CD4+, and CD8+ T cells. It is upregulated in a variety of cancers. CXCR6 mediates the recruitment of immune cells to sites of inflammation and tissue damage. It also promotes NK cell memory and tolerance to NKT cell-mediated graft rejection. CXCR6 enhances the invasiveness of both tumor cells and glial precursor cells. In addition, it can function as a cellular receptor for select variants of HIV types 1 and 2. Rat CXCR6 shares 72% and 88% amino acid sequence identity with human and mouse CXCR6, respectively.

Entrez Gene IDs

10663 (Human); 80901 (Mouse); 100124593 (Rat)

Alternate Names

Bonzo; BONZOG-protein coupled receptor bonzo; CD186 antigen; CD186; CDw186; chemokine (C-X-C motif) receptor 6; C-X-C chemokine receptor type 6; CXCR6; CXC-R6; CXCR-6; G protein-coupled receptor; STRL33; STRL33G-protein coupled receptor STRL33; TYMSTRCD186