Rat GFR alpha-1/GDNF R alpha-1 Biotinylated Antibody
Rat GFR alpha-1/GDNF R alpha-1 Biotinylated Antibody Summary
Asp25-Leu445
Accession # Q62997
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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GDNF and GFR alpha ‑1 in Rat Dorsal Root Ganglia. GDNF and GFRa-1 were detected in perfusion fixed frozen sections of rat dorsal root ganglia using 10 µg/mL Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF-212-NA) and 15 µg/mL Goat AntiRat Anti-GFRa-1 Biotinylated Antigen Affinity-purified Poly-clonal Antibody (red; Catalog # BAF560). Tissue was incubated with primary antibodies overnight at 4 °C. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GFR alpha-1/GDNF R alpha-1
Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF-beta superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha - (GFR alpha -1) or GFR alpha -2). GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase. Rat GFR alpha -1 cDNA encodes a 468 amino acid (aa) residue protein with an N‑terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% amino acid identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.
- Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
- Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
- Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
Product Datasheets
Citations for Rat GFR alpha-1/GDNF R alpha-1 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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GDNF availability determines enteric neuron number by controlling precursor proliferation.
Authors: Gianino S, Grider JR, Cresswell J, Enomoto H, Heuckeroth RO
Development, 2003-05-01;130(10):2187-98.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
TGFbeta induces GDNF responsiveness in neurons by recruitment of GFRalpha1 to the plasma membrane.
Authors: Peterziel H, Unsicker K, Krieglstein K
J. Cell Biol., 2002-10-07;159(1):157-67.
Species: Chicken, Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
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