Rat Glycine R Pan Specific Antibody

Catalog #: PPS009 Datasheet / COA / SDS

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PPS009

Detection of Rat Glycine R by Western Blot.

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All Glycine R Antibodies

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Rat Glycine R Pan Specific Antibody Summary

Species Reactivity

Rat

Specificity

Specific for the ~48 kDa alpha 1 and alpha 2 subunits of the Glycine Receptor in Western blots of rat spinal cord, brain stem and cell extracts. Immunolabeling is blocked by preadsorption of antibody with the peptide immunogen. This antibody does not recognize other Glycine Receptor subunits.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Peptide from the N-terminus region of the alpha 1 subunit of the rat Glycine Receptor

Formulation

Lyophilized in 5 mM ammonium bicarbonate

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Immunohistochemistry

1:1000 dilution

Frozen sections; unpublished observations, 1:1000

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Rat Glycine R by Western Blot. Western blot of 10 μg of rat spinal cord. As shown in the autoradiograph, the Anti-Glycine Receptor antibody is specific for the ~48 kDa subunits recognizing both the alpha 1 and alpha 2 subunits of the Glycine Receptor.

Reconstitution Calculator

Preparation and Storage

Reconstitution

This antibody should be reconstituted in 100 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

The lyophilized product is stable at ≤- 20° C for at least 1 year. After reconstitution the antibody should be aliquoted and stored at ≤ -20° C.

Background: Glycine R

The rat glycine receptor (GlyR) chloride channel is a member of the nicotinic acetylcho-line receptor family of ligand-gated ion channels. Its glycine-mediated activation results in an influx of chlorine ions that hyperpolarize and desensitize neuronal postsynaptic membranes. The receptor’s multimeric makeup is unclear. It may be either a three or five subunit homo- or heteromultimer. To date, there are five rat GlyR subunit types. Four are 48-53 kDa alpha -type subunits, and one is a 58 kDa beta -type subunit. Alternate splicing exists for alpha 1 and alpha 2 subunit types. Alpha-type subunits may form homotrimers; however, their function may not be neurotransmission but rather non-synaptic cell-cell communication. Alpha1-beta multimers may be the principal synaptic GlyR in adults.

Alternate Names

Glycine R