Rat Hepassocin/FGL1 Antibody

Catalog #: MAB11051 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11051-100
MAB11051-SP

Rat Hepassocin/FGL1 ELISA Standard Curve.

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Product Details

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat Hepassocin/FGL1 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat Hepassocin/FGL1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 160915

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Hepassocin/FGL1
Asp25-Val314
Accession # Q5M8C6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Rat Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB11052).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Rat Hepassocin/FGL1 ELISA Standard Curve. Recombinant Rat Hepassocin/FGL1 protein was serially diluted 2-fold and captured by Mouse Anti-Rat Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB11052) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Rat Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB11051) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hepassocin/FGL1

Hepassocin, also known as hepatocyte-derived fibrinogen-related protein 1 (HFREP-1), is a liver-specific secreted protein containing one fibrinogen C-terminal domain.

Long Name

Fibrinogen Like 1

Entrez Gene IDs

2267 (Human); 246186 (Rat); 102118020 (Cynomolgus Monkey)

Alternate Names

FGL1; fibrinogen-like 1; fibrinogen-like protein 1; Fibrinogen-like-protein 1; FREP1; Hepassocin; hepatocellular carcinoma-related sequence; Hepatocyte-derived fibrinogen-related protein 1; HFREP-1; HFREP1MGC12455; HP-041; LFIRE1; LFIRE-1; Liver fibrinogen-related protein 1