Rat Hepassocin/FGL1 Antibody

Catalog # Availability Size / Price Qty
AF1105-SP Ships in 5-7 Days25 ug / $149
AF1105 In Stock100 ug / $429
Bulk Orders
Hepassocin/FGL1 in Rat Liver.
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Product Details
Citations (2)
FAQs
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Rat Hepassocin/FGL1 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Hepassocin/FGL1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Hepassocin/FGL1
Asp25-Val314
Accession # Q5M8C6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat Hepassocin/FGL1
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Hepassocin/FGL1 antibody in Rat Liver by Immunohistochemistry (IHC-Fr). View Larger

Hepassocin/FGL1 in Rat Liver. Hepassocin/FGL1 was detected in perfusion fixed frozen sections of rat liver using 15 µg/mL Goat Anti-Rat Hepassocin/FGL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1105) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
In Stock
2 mL / $12
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hepassocin/FGL1

Hepassocin, also known as hepatocyte-derived fibrinogen-related protein 1 (HFREP-1), is a liver-specific secreted protein belonging to the fibrinogen superfamily, whose members share a fibrinogen domain at their C-termini. Hepassocin may play a role in the development of hepatocellular carcinomas. Hepassocin is a disulfide-linked homodimeric protein with a C-terminal fibrinogen domain. It is secreted by the liver and functions as a mitogen for hepatocytes. Hepassocin is upregulated during liver regeneration following partial hepatectomy.

Long Name
Fibrinogen Like 1
Entrez Gene IDs
2267 (Human); 246186 (Rat); 102118020 (Cynomolgus Monkey)
Alternate Names
FGL1; fibrinogen-like 1; fibrinogen-like protein 1; Fibrinogen-like-protein 1; FREP1; Hepassocin; hepatocellular carcinoma-related sequence; Hepatocyte-derived fibrinogen-related protein 1; HFREP-1; HFREP1MGC12455; HP-041; LFIRE1; LFIRE-1; Liver fibrinogen-related protein 1

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Citations for Rat Hepassocin/FGL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Recombinant human hepassocin stimulates proliferation of hepatocytes in vivo and improves survival in rats with fulminant hepatic failure.
    Authors: Li CY, Cao CZ, Xu WX, Cao MM, Yang F, Dong L, Yu M, Zhan YQ, Gao YB, Li W, Wang ZD, Ge CH, Wang QM, Peng RY, Yang XM
    Gut, 2009-11-01;59(6):817-26.
    Species: Human, Mouse, Rat
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  2. Fibrinogen-like protein 1, a hepatocyte derived protein is an acute phase reactant.
    Authors: Liu Z, Ukomadu C
    Biochem. Biophys. Res. Commun., 2007-11-26;365(4):729-34.
    Species: Rat
    Sample Types: Serum
    Applications: ELISA Development

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody

WB,Secondary

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