Rat IFN-beta Antibody

Catalog #: 33400-1
1 Citation Datasheet / COA / SDS

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33400-1

All IFN-beta Antibodies

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Citations (1)

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Rat IFN-beta Antibody Summary

Species Reactivity

Rat

Specificity

Neutralizes both natural and recombinant rat interferon beta. Cross-reactivity with human interferon beta has not been tested.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant rat IFN-beta

Formulation

0.5 ml Phosphate-buffered saline (PBS) + 125 mM trehalose prior to lyophilization.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

One neutralization unit is the amount of antiserum required to neutralize one unit of recombinant rat interferon beta (rat IFN-beta ) to a 50% endpoint (one unit corresponds to approximately 15 pg of purified rat IFN-beta ). Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. As there is no international rat IFN-beta standard,the units are determined with respect to the international reference standard for mouse interferon beta (mouse IFN-beta ) provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material is prepared specifically for effective neutralization of rat IFN-beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Dissolve contents of the vial by injection of 0.5 ml sterile distilled water.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Upon receipt, this product is stable at 4°C until the expiration date listed above. After reconstitution, the contents can be safely stored at 4°C for one month or at -20°C for one year for retention of full activity.When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Add 0.02% sodium azide to prevent bacterial growth.

Background: IFN-beta

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

References

Ruuls, S.R. et al. (1996) J. Immunol. 157: 5721;
Zou, L.P. et al. 1999. Neuroscience Res. 56: 123

Long Name

Interferon beta

Entrez Gene IDs

3456 (Human); 15977 (Mouse); 24481 (Rat)

Alternate Names

Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Rat IFN-beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Lipid-specific IgMs induce antiviral responses in the CNS: implications for progressive multifocal leukoencephalopathy in multiple sclerosis
Authors: L Hayden, T Semenoff, V Schultz, SF Merz, KJ Chapple, M Rodriguez, AE Warrington, X Shi, CS McKimmie, JM Edgar, K Thümmler, C Linington, M Pingen
Acta Neuropathol Commun, 2020-08-13;8(1):135.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization