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Rat IgG Antibody

Catalog #: 6-001-A
34 Citations 5 Reviews Datasheet / COA / SDS
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6-001-A
R&D Systems Antibodies
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Product Details
Citations (34)
FAQs
Supplemental Products
Reviews (5)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat IgG Antibody Summary

Specificity
This product has been purified for use as normal rat IgG.
Source
Polyclonal Rat IgG
Immunogen
NA
Formulation
10 mM Sodium Phosphate, 0.85% (w/v) Sodium Chloride, pH 7.2 (PBS) with 5% trehalose and 0.05% (w/v) Sodium Azide
Label
Unconjugated

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute with sterile PBS. If 0.5 mL of PBS is used, the antibody concentration will be 2 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Rat IgG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

34 Citations: Showing 1 - 10
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  1. Foam Cell Formation In Vivo Converts Macrophages to a Pro-Fibrotic Phenotype.
    Authors: Thomas A, Eijgelaar W, Daemen M, Newby A
    PLoS ONE, 2015-07-21;10(7):e0128163.
  2. Tumour stroma-derived Lipocalin-2 promotes breast cancer metastasis
    Authors: B Ören, J Urosevic, C Mertens, J Mora, M Guiu, RR Gomis, A Weigert, T Schmid, S Grein, B Brüne, M Jung
    J Pathol, 2016-04-29;0(0):.
  3. High mobility group box-1 protein promotes astrocytic CCL5 production through the MAPK/NF-?B pathway following spinal cord injury
    Authors: Chi, G;Lu, J;He, T;Wang, Y;Zhou, X;Zhang, Y;Qiu, L;
    Scientific reports
    Species: Rat
    Sample Types: In Vivo
    Applications: In vivo assay
  4. High glucose intake exacerbates experimental autoimmune prostatitis through mitochondrial reactive oxygen species-dependent TGF-? activation-mediated Th17 differentiation
    Authors: Niu, D;Yue, SY;Wang, X;Li, WY;Zhang, L;Du, HX;Liang, CZ;
    International immunopharmacology
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Protective effects of macrophage-specific integrin ?5 in myocardial infarction are associated with accentuated angiogenesis
    Authors: Li, R;Chen, B;Kubota, A;Hanna, A;Humeres, C;Hernandez, SC;Liu, Y;Ma, R;Tuleta, I;Huang, S;Venugopal, H;Zhu, F;Su, K;Li, J;Zhang, J;Zheng, D;Frangogiannis, NG;
    Nature communications
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Control
  6. CD1d-dependent natural killer T-cells inactivation aggravates sepsis-induced myocardial injury via T lymphocytes infiltration and IL-6 production in mice
    Authors: Chen, LR;Hui-Yang, ;Lu-Liu, ;Wang, XX;Zhang, XM;Wang, HX;
    International immunopharmacology
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. LOTUS suppresses amyloid beta -induced dendritic spine elimination through the blockade of amyloid beta binding to PirB
    Authors: Yuki Kawaguchi, Junpei Matsubayashi, Yutaka Kawakami, Ryohei Nishida, Yuji Kurihara, Kohtaro Takei
    Molecular Medicine
  8. IL6 supports long-term expansion of hepatocytes in vitro
    Authors: R Guo, M Jiang, G Wang, B Li, X Jia, Y Ai, S Chen, P Tang, A Liu, Q Yuan, X Xie
    Nature Communications, 2022-11-29;13(1):7345.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Isotype Control
  9. Loss of IL-33 enhances elastase-induced and cigarette smoke extract-induced emphysema in mice
    Authors: D Morichika, A Taniguchi, N Oda, U Fujii, S Senoo, J Itano, A Kanehiro, Y Kitaguchi, M Yasuo, M Hanaoka, T Satoh, S Akira, K Kiura, Y Maeda, N Miyahara
    Respiratory Research, 2021-05-15;22(1):150.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Isotype Control
  10. Obesity-Activated Lung Stromal Cells Promote Myeloid Lineage Cell Accumulation and Breast Cancer Metastasis
    Authors: Hillers-Ziemer LE, Williams AE, Janquart A et al.
    Cancers (Basel)
  11. Wnt signaling contributes to withdrawal symptoms from opioid receptor activation induced by morphine exposure or chronic inflammation
    Authors: Mingzheng Wu, Zehua Li, Lei Liang, Pingchuan Ma, Dong Cui, Peng Chen et al.
    Pain
  12. Combination of Mycobacterium indicus pranii and heat-induced promastigotes cures drug-resistant Leishmania infection: Critical role of IL-6 producing classical dendritic cells
    Authors: S Dey, D Mukherjee, SS Sultana, S Mallick, A Dutta, J Ghosh, A Hussain, B Sarkar, S Mandal, P Patra, B Saha, C Pal
    Infect. Immun., 2020-05-20;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization Control
  13. Hepatospecific ablation of p38alpha MAPK governs liver regeneration through modulation of inflammatory response to CCl4-induced acute injury
    Authors: M Fortier, M Cadoux, N Boussetta, S Pham, R Donné, JP Couty, C Desdouets, S Celton-Mor
    Sci Rep, 2019-10-10;9(1):14614.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  14. The Oral Mouse Microbiome Promotes Tumorigenesis in Oral Squamous Cell Carcinoma
    Authors: Philip Stashenko, Susan Yost, Yoonhee Choi, Theodora Danciu, Tsute Chen, Subbiah Yoganathan et al.
    mSystems
  15. Batroxobin accelerated tissue repair via neutrophil extracellular trap regulation and defibrinogenation in a murine ischemic hindlimb model
    Authors: Haruchika Masuda, Atsuko Sato, Tomoko Shizuno, Keiko Yokoyama, Yusuke Suzuki, Masayoshi Tokunaga et al.
    PLOS ONE
  16. Caspase-1 induces smooth muscle cell growth in hypoxia-induced pulmonary hypertension
    Authors: Camilla Udjus, Fadila T. Cero, Bente Halvorsen, Dina Behmen, Cathrine R. Carlson, Bård A. Bendiksen et al.
    American Journal of Physiology-Lung Cellular and Molecular Physiology
  17. Methotrexate Chemotherapy Induces Persistent Tri-glial Dysregulation that Underlies Chemotherapy-Related Cognitive Impairment
    Authors: Erin M. Gibson, Surya Nagaraja, Alfonso Ocampo, Lydia T. Tam, Lauren S. Wood, Praveen N. Pallegar et al.
    Cell
  18. Repeated water avoidance stress induces visceral hypersensitivity; role of IL-1, IL-6 and peripheral corticotropin-releasing factor
    Authors: T Nozu, S Miyagishi, R Nozu, K Takakusaki, T Okumura
    J. Gastroenterol. Hepatol, 2017-12-01;0(0):.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  19. IL-17A Produced by Innate Lymphoid Cells Is Essential for Intestinal Ischemia-Reperfusion Injury
    Authors: M Geha, MG Tsokos, RE Bosse, T Sannikova, Y Iwakura, JJ Dalle Lucc, R De Waal Ma, GC Tsokos
    J. Immunol., 2017-09-06;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  20. T Cell–Derived Granulocyte-Macrophage Colony-Stimulating Factor Contributes to Dry Eye Disease Pathogenesis by Promoting CD11b+ Myeloid Cell Maturation and Migration
    Authors: Thomas H. Dohlman, Julia Ding, Reza Dana, Sunil K. Chauhan
    Investigative Opthalmology & Visual Science
  21. Class A Scavenger Receptor Exacerbates Osteoclastogenesis by an Interleukin-6-Mediated Mechanism through ERK and JNK Signaling Pathways
    Authors: Shuyu Guo, Yuanyuan Ni, Jingjing Ben, Yang Xia, Tingting Zhou, Dongyue Wang et al.
    International Journal of Biological Sciences
  22. CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation
    Proc Natl Acad Sci USA, 2016-08-01;113(33):E4847-56.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  23. Tumor-infiltrating monocytes/macrophages promote tumor invasion and migration by upregulating S100A8 and S100A9 expression in cancer cells
    Authors: SY Lim, AE Yuzhalin, AN Gordon-Wee, RJ Muschel
    Oncogene, 2016-04-18;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  24. E-Selectin Mediates Immune Cell Trafficking in Corneal Transplantation
    Authors: Thomas H. Dohlman, Antonio Di Di Zazzo, Masahiro Omoto, Jing Hua, Julia Ding, Pedram Hamrah et al.
    Transplantation
  25. Dexamethasone Regulates EphA5, a Potential Inhibitory Factor with Osteogenic Capability of Human Bone Marrow Stromal Cells
    Authors: Tsuyoshi Yamada, Toshitaka Yoshii, Hiroaki Yasuda, Atsushi Okawa, Shinichi Sotome
    Stem Cells International
  26. IL17 Mediates Pelvic Pain in Experimental Autoimmune Prostatitis (EAP).
    Authors: Murphy S, Schaeffer A, Done J, Wong L, Bell-Cohn A, Roman K, Cashy J, Ohlhausen M, Thumbikat P
    PLoS ONE, 2015-05-01;10(5):e0125623.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization Control
  27. Systemic inflammatory response elicited by superantigen destabilizes T regulatory cells, rendering them ineffective during toxic shock syndrome.
    Authors: Tilahun A, Chowdhary V, David C, Rajagopalan G
    J Immunol, 2014-08-04;193(6):2919-30.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization Control
  28. Distinct mechanisms of induction of hepatic growth hormone resistance by endogenous IL-6, TNF-alpha, and IL-1beta.
    Authors: Zhao Y, Xiao X, Frank S, Lin H, Xia Y
    Am J Physiol Endocrinol Metab, 2014-06-03;307(2):E186-98.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  29. Wnt5a-Rac1-NF-kappaB homeostatic circuitry sustains innate immune functions in macrophages.
    Authors: Naskar D, Maiti G, Chakraborty A, Roy A, Chattopadhyay D, Sen M
    J Immunol, 2014-04-04;192(9):4386-97.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. CCR6 is required for epidermal trafficking of gammadelta-T cells in an IL-23-induced model of psoriasiform dermatitis.
    Authors: Mabuchi T, Singh T, Takekoshi T, Jia G, Wu X, Kao M, Weiss I, Farber J, Hwang S
    J Invest Dermatol, 2012-08-16;133(1):164-71.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  31. Relationship between complement membrane attack complex, chemokine (C-C motif) ligand 2 (CCL2) and vascular endothelial growth factor in mouse model of laser-induced choroidal neovascularization.
    Authors: Liu J, Jha P, Lyzogubov VV, Tytarenko RG, Bora NS, Bora PS
    J. Biol. Chem., 2011-04-22;286(23):20991-1001.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  32. Interleukin-10 production by tumor infiltrating macrophages plays a role in Human Papillomavirus 16 tumor growth
    Authors: Aline Bolpetti, João S Silva, Luisa L Villa, Ana Paula Lepique
    BMC Immunology
  33. Vaccinia virus inoculation in sites of allergic skin inflammation elicits a vigorous cutaneous IL-17 response.
    Authors: Oyoshi MK, Elkhal A, Kumar L, Scott JE, Koduru S, He R, Leung DY, Howell MD, Oettgen HC, Murphy GF, Geha RS
    Proc. Natl. Acad. Sci. U.S.A., 2009-08-17;106(35):14954-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  34. Reduction in human epidermal Langerhans cells with age is associated with decline in CXCL14-mediated recruitment of CD14+ monocytes
    Authors: Hasegawa T, Feng Z, Yan Z et al.
    J. Invest. Dermatol.

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Reviews for Rat IgG Antibody

Average Rating: 4.8 (Based on 5 Reviews)

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Normal Rat IgG Control
By Anonymous on 07/12/2021
Application: IHC Sample Tested: Thighbone (femur) Species: Mouse
Immunohistochemistry Normal Rat IgG Control 6-001-A
Normal Rat IgG Control
By Anonymous on 04/28/2021
Application: IHC Sample Tested: Testis tissue Species: Mouse
Immunohistochemistry Normal Rat IgG Control 6-001-A
Normal Rat IgG Control
By Anonymous on 09/28/2019
Application: Func Sample Tested: Recombinant protein Species: Rat
Functional Assay Normal Rat IgG Control 6-001-A
Normal Rat IgG Control
By Anonymous on 03/11/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult lung Species: Human

no background with and without antigen retrieval

Immunocytochemistry/Immunofluorescence Normal Rat IgG Control 6-001-A
Normal Rat IgG Control
By Anonymous on 09/08/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: cornea Species: Mouse