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Rat IgG1 Fluorescein‑conjugated Control Antibody

Catalog #: IC005F
3 Citations Datasheet / COA / SDS
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IC005F
R&D Systems Antibodies
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Citations (3)
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Rat IgG1 Fluorescein‑conjugated Control Antibody Summary

Specificity
Selected for use as rat IgG1 isotype control for flow cytometry applications.
Source
Monoclonal Rat IgG1 Clone # 43414
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G1
Alternate Names
IgG1; Igh-4; RGD1359539; VH7183

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Citations for Rat IgG1 Fluorescein‑conjugated Control Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Toll-like receptor 4 and macrophage scavenger receptor 1 crosstalk regulates phagocytosis of a fungal pathogen
    Authors: Onyishi, CU;Desanti, GE;Wilkinson, AL;Lara-Reyna, S;Frickel, EM;Fejer, G;Christophe, OD;Bryant, CE;Mukhopadhyay, S;Gordon, S;May, RC;
    Nature communications
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  2. Impaired B cell receptor signaling is responsible for reduced TACI expression and function in X-linked immunodeficient mice.
    Authors: Uslu K, Coleman A, Allman W, Katsenelson N, Bram R, Alugupalli K, Akkoyunlu M
    J Immunol, 2014-03-19;192(8):3582-95.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. FcgammaRIIB signals inhibit BLyS signaling and BCR-mediated BLyS receptor up-regulation.
    Authors: Crowley JE, Stadanlick JE, Cambier JC, Cancro MP
    Blood, 2008-09-12;113(7):1464-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control

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