Rat IL-5 Antibody

Catalog #: AF545
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF545
AF545-SP

Cell Proliferation Induced by IL‑5 and Neutralization by Rat IL‑5 Antibody.

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All IL-5 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat IL-5 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat IL‑5 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IL‑5 is observed and 5% cross‑reactivity with recombinant human IL‑5 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat IL-5
Met20-Val132
Accession # Q08125

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Rat IL‑5 (Catalog # 545-RL)

Immunocytochemistry

5-15 µg/mL

Immersion fixed rat splenocytes

Neutralization

Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 2-6 µg/mL in the presence of 1.25 ng/mL Recombinant Rat IL‑5.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑5 and Neutralization by Rat IL‑5 Antibody. Recombinant Rat IL-5 (Catalog # 545-RL) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF545). The ND₅₀ is typically 2-6 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-5

Interleukin 5 is a T cell-derived factor that promotes the proliferation, differentiation and activation of eosinophils. In mice, IL-5 has also been shown to be a growth and differentiation factor for B cells. Various names previously used to describe IL-5 include: T cell replacing factor (TRF), B cell growth factor II (BCGFII), B cell differentiation factor μ (BCDF μ), eosinophil differentiation factor (EDF) and eosinophil colony-stimulating factor (Eo-CSF). Biologically active IL-5 is a disulfide-linked homodimer. The cDNAs formurine, human and rat IL-5 encode precursor proteins with signal peptides that are cleaved to form mature proteins containing 113, 115, and 113 amino acid residues, respectively. Rat IL-5 is 94% and 70% identical to mouse and human IL-5, respectively. The genes for human and mouse IL-5 have been mapped to chromosome 5 and chromosome 11, respectively; closely linked tothe genes for IL-3, IL-4 and GM-CSF.

IL-5 exerts its activity on target cells by binding to specific cell surface receptors. The functional high-affinity receptor for human IL-5 has been shown to be composed of a low-affinity IL-5 binding alpha -subunit and a non-binding common beta -subunit that is sharedwith the high-affinity receptors for GM-CSF and IL-3.

References

Karlen, S. et al. (1998) Intern. Rev. Immunol. 16:227.
Lalani, T. et al. (1999) Annals Allergy Asthma Immunol. 82:317.
Takatsa, K. (1998) Cytokine and Growth Factor Reviews 9:25.

Long Name

Interleukin 5

Entrez Gene IDs

3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)

Alternate Names

BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I

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Citation for Rat IL-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Effects of Energy Drink Acute Assumption in Gastrointestinal Tract of Rats
Authors: M Nasi, A De Gaetano, G Carnevale, L Bertoni, V Selleri, G Zanini, A Pisciotta, S Caramaschi, L Reggiani B, A Farinetti, A Cossarizza, M Pinti, A Manenti, AV Mattioli
Nutrients, 2022-05-04;14(9):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC