Rat IL-9R Antibody

Catalog #: AF4896 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4896
AF4896-SP

All IL-9R Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat IL-9R Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat IL-9 R in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant mouse IL-9 R is observed and approximately 10% cross-reactivity with recombinant human IL-9 R is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat IL‑9 R
Val38-Ala270
Accession # EDM04022

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Rat IL‑9 R Subunit (Catalog # 4896-RR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-9R

The IL-9 receptor alpha protein is a member of the type I cytokine receptor family. It is a 62 kDa IL-9 receptor subunit (previously Gfi-2, designated CD129) that binds IL-9 and pairs with the 64 kDa cytokine receptor common gamma -chain to allow cell signaling (1‑3). The 467 amino acid (aa) rat IL-9 R alpha precursor is predicted to contain a 37 aa signal sequence, a 232 aa extracellular domain (ECD) with four conserved cysteine residues in its N-terminal region, a fibronectin type III domain and a WSXWS motif, a 21 aa transmembrane (TM) domain, and a 177 aa cytoplasmic domain with a Box 1 JAK-binding motif (1, 2, 4). A potential isoform contains a 36 aa sequence that is substituted for the TM and cytoplasmic domains (5). The ECD of rat IL-9 R alpha shares 86%, 63%, 66%, 63% and 59% aa identity with the ECD of mouse, human, equine, canine and bovine IL-9 R alpha, respectively. Mast cells, germinal center and B-1b B cells, T cell blasts, and myeloid progenitors express IL-9 R (1, 6‑8). The primary functions of IL-9 have been difficult to determine due to the cross-functionality and cross-induction of cytokines. IL-9 production by regulatory T cells (T-regs), however, is crucial for mast cell recruitment and differentiation, which in turn is crucial for tolerance of allografts (7). Genetic deletion and transgenic expression of IL-9 and other cytokines has also demonstrated the role of IL-9 in mastocytosis, eosinophilia and B cell infiltration of the lung in a mouse asthma-like model, while IL-9 induction of IL-13 is responsible for eosinophil recruitment and mucous upregulation (8‑10).

References