Rat MAG/Siglec-4a Antibody

Catalog #: MAB538 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB538
MAB538-SP

Cell Adhesion Mediated by MAG/Siglec-4a and Neutralization by Rat MAG/Sigle-4a Antibody.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat MAG/Siglec-4a Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat MAG/Siglec‑4a in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 118423

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat MAG/Siglec‑4a
Gly20-Pro516
Accession # P07722

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Rat MAG/Siglec‑4a Fc Chimera (Catalog # 538-MG)

Neutralization

Measured by its ability to neutralize MAG/Siglec-4a-mediated adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. At 40 μg/mL, Mouse Anti-Rat MAG/Siglec-4a Monoclonal Antibody will neutralize approximately 60-85% of Recombinant Rat MAG/Siglec-4a Fc Chimera-dependent adhesion of human red blood cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Adhesion Mediated by MAG/Siglec-4a and Neutralization by Rat MAG/Sigle-4a Antibody. Recombinant Rat MAG/Siglec-4a Fc Chimera (Catalog # 538-MG), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Rat MAG/Siglec-4a Fc Chimera (6 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat MAG/Siglec-4a Monoclonal Antibody (Catalog # MAB538). At 40 µg/mL, this antibody will neutralize approximately 60-85% of Recombinant Rat MAG/Siglec-4a-dependent adhesion of human red blood cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MAG/Siglec-4a

MAG (Myelin-Associated Glycoprotein), a type I transmembrane glycoprotein containing five Ig-like domains in its extracellular domain is an adhesion molecule belonging to the immunoglobin superfamily. Within this superfamily, MAG, CD22, CD33, Schwann cell myelin protein, and sialoadhesin which bind specifically to cell‑surface glycan containing sialic acid residues define the I-type sialyl lectin subgroup, also called the sialoadhesin family. Sialoadhesins mediate diverse biological processes through recognition of specific sialyted glycans on cell surface. MAG is expressed on myelinating oligodenrocytes and Schwann cells, and preferentially recognizes alpha 2, 3-linked sialic acid on O-linked glycans and gangliosides. MAG exists as two isoforms which differ in the sequence and length of the cytoplasmic tail. The large form (71 kDa) and small form (67 kDa) arise from alternative spliced mRNAs. Although MAG might encounter haematopoietic cells and lymphocytes under pathologic conditions, it would normally be expected to interact with neuronal cells. It has been shown that MAG promotes axonal growth from neonatal DRG neurons and embryonic spinal neurons, but is a potent inhibitor of axonal re-growth from adult DRG and postnatal cerebellar neurons. MAG plays an important role in the interaction between axons and myelin. A soluble form of MAG containing the extracellular domain is released from myelin in large quantities and identified in normal human tissues and in tissues from patients with neurological disorders. This soluble MAG might contribute to the lack of CNS neuron regeneration after injury.

References

Kelm, S. et al. (1994) Current Biology 4:965.
McKerracher, L. et al. (1994) Neuron 13:805.
Tang, S. et al. (1997) Molecular and Cellular Neuroscience 9:333.
Cai, D. et al. (1999) Neuron 22:89.

Long Name

Myelin-associated Glycoprotein

Entrez Gene IDs

4099 (Human); 17136 (Mouse); 29409 (Rat)

Alternate Names

GMAsialic acid binding Ig-like lectin 4A; MAG; myelin associated glycoprotein; myelin-associated glycoprotein; sialic acid-binding immunoglobulin-like lectin 4A; Siglec4a; Siglec-4a; S-MAG