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Rat Neurexin 1 beta /NXRN1b Biotinylated Antibody

Catalog #: BAF4524 Datasheet / COA / SDS
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BAF4524
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Rat Neurexin 1 beta /NXRN1b Biotinylated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Neurexin 1 beta in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant rat Neurexin 1 alpha is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Neurexin 1 beta isoform 1
Ala47-Thr392
Accession # Q63373
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat Neurexin 1 beta /NXRN1b

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurexin 1 beta/NXRN1b

Neurexin 1 beta is an 80 kDa member of the Neurexin family of proteins. It is expressed on neuronal presynaptic membranes and contributes to synapse formation through binding to postsynaptic neuroligins. An alternate start site in the Neurexin 1 gene generate an alpha - (long) and beta - (short) isoform. Mature rat Neurexin 1 beta is a type I transmembrane glycoprotein that is 468 amino acids (aa) in length. It has a 346 aa extracellular region (aa 47‑392) that contains one laminin-like G domain (aa 91‑264). Within the G domain, the presence or absence of a splice site (aa 201‑230) materially impacts neuroligin binding. Over aa 47‑392, rat Neurexin 1 beta is 100% and 98% aa identical to mouse and human Neurexin 1 beta, respectively.

Entrez Gene IDs
9378 (Human); 18189 (Mouse); 60391 (Rat)
Alternate Names
Hs.22998; Neurexin 1 beta; NXR1B; NXRN1b; PTHSL2; SCZD17

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