Rat Neuropilin-1 Antibody

Catalog #: MAB5661
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5661
MAB5661-SP

All Neuropilin-1 Antibodies

Product Details

Citations (3)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat Neuropilin-1 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant rat Neuropilin-2 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 130604

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1
Met1-Asp854 (Lys811Arg, Pro812-Gly828 del), predicted
Accession # Q9QWJ9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Rat Neuropilin‑1/BDCA4 Fc Chimera (Catalog # 566-N1)

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of rat embryo (E15)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuropilin-1

Neuropilin-1 (Npn-1, previously neuropilin; also CD304) is a 130‑140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1‑4). The mature 901 amino acid (aa) rat Npn-1 contains a 623 aa extracellular domain (ECD) that shares 98% aa identity with mouse and 93% aa identity with human, equine, bovine and canine Npn-1 (3, 4). The ECD contains two N-terminal CUB domains, two F5/8 type C domains with homology to coagulation factors V and VIII and a MAM (meprin) domain. In mouse and human, splice variants that lack the TM domain have been described and are either proven or presumed to be soluble antagonists (1, 5‑7). The sema domains of Class III secreted semaphorins such as Sema3A bind Npn-1 CUB domains (8). The heparin-binding forms of VEGF (VEGF₁₆₅, VEGF-B and VEGF-E), PlGF (PlGF2), and the C-terminus of Sema3 bind the F5/8 type C domains (8, 9). Npn-1 and Npn-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both neuropilins act as co-receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (10). However, only Npn-1 binds Sema3A, and only Npn-2 binds Sema3F (1). Both are co-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF₁₆₅ binding (1). Sema3A signaling can be blocked by VEGF₁₆₅, which has higher affinity for Npn-1 (11). Npn-1 is preferentially expressed in developing or remodeling arteries (1, 2). Npn-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation (12).

References

Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584.
Gu, C. et al. (2003) Dev. Cell 5:45.
He, Z. and M. Tessier-Lavigne (1997) Cell 90:739.
Soker, S. et al. (1998) Cell 92:735.
Gagnon, M.L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2573.
Cackowski, F.C. et al. (2004) Genomics 84:82.
Rossignol, M. et al. (2000) Genomics 70:211.
Gu, C. et al. (2002) J. Biol. Chem. 277:18069.
Mamluk, R. et al. (2002) J. Biol. Chem. 277:24818.
Yaron, A. et al. (2005) Neuron 45:513.
Narazaki, M. and G. Tosato (2006) Blood 107:3892.
Tordjman, R. et al. (2002) Nat. Immunol. 3:477.

Entrez Gene IDs

8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)

Alternate Names

BDCA4; BDCA-4; CD304 antigen; CD304; DKFZp686A03134; DKFZp781F1414; neuropilin 1; Neuropilin1; Neuropilin-1; NP1; NRP; NRP1; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165R

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Product Datasheet

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Related Research Areas

Citations for Rat Neuropilin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Neuropilin-1 is a receptor for extracellular miRNA and AGO2/miRNA complexes and mediates the internalization of miRNAs that modulate cell function
Authors: GJ Prud'homme, Y Glinka, Z Lichner, GM Yousef
Oncotarget, 2016-10-18;7(42):68057-68071.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC
Nephric duct insertion requires EphA4/EphA7 signaling from the pericloacal mesenchyme.
Authors: Weiss A, Airik R, Bohnenpoll T, Greulich F, Foik A, Trowe M, Rudat C, Costantini F, Adams R, Kispert A
Development, 2014-09-01;141(17):3420-30.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Semaphorin 3A suppresses VEGF-mediated angiogenesis yet acts as a vascular permeability factor.
Authors: Acevedo LM, Barillas S, Weis SM, Gothert JR, Cheresh DA
Blood, 2008-01-07;111(5):2674-80.
Species: Mouse
Sample Types: In Vivo, Whole Tissue
Applications: IHC-Fr, Neutralization