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Rat Reg3B Antibody

Catalog #: MAB1996
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1996
MAB1996-SP
Reg3B in Rat Pancreas.
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat Reg3B Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Reg3B in direct ELISAs and Western blots. Does not cross-react with recombinant mouse Reg1A, 1B, 2A, recombinant rat Reg3, or recombinant human Reg4.
Source
Monoclonal Mouse IgG1 Clone # 293523
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant rat Reg3B
Glu27-Gly175
Accession # P25031
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Rat Reg3B under non-reducing conditions only
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Reg3B antibody in Rat Pancreas by Immunohistochemistry (IHC-Fr). View Larger

Reg3B in Rat Pancreas. Reg3B was detected in perfusion fixed frozen sections of rat pancreas using Mouse Anti-Rat Reg3B Monoclonal Antibody (Catalog # MAB1996) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of exocrine cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reg3B

Reg3B, also named Pancreatitis-Associated Protein (PAP) and peptide 23, belongs to the Regenerating gene family (Reg), within the superfamily of C-type lectins. It has been classified as a member of the type III subclass of the Reg family. Reg3B has anti-apoptotic effects on pancreatic acinar cells and is a novel motor and sensory neuron survival factor.

Long Name
Regenerating Islet-derived IIIb
Entrez Gene IDs
18489 (Mouse); 24618 (Rat)
Alternate Names
PAP1; Reg3B

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Citations for Rat Reg3B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Reg3alpha and Reg3beta Expressions Followed by JAK2/STAT3 Activation Play a Pivotal Role in the Acceleration of Liver Hypertrophy in a Rat ALPPS Model
    Authors: N Otsuka, M Yoshioka, Y Abe, Y Nakagawa, H Uchinami, Y Yamamoto
    Int J Mol Sci, 2020-06-07;21(11):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  2. Nerve Injury-Induced Neuronal PAP-I Maintains Neuropathic Pain by Activating Spinal Microglia
    Authors: J Li, H Shi, H Liu, F Dong, Z Liu, Y Lu, L Chen, L Bao, X Zhang
    J. Neurosci., 2019-11-19;40(2):297-310.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  3. Dietary copper-fructose interactions alter gut microbial activity in male rats
    Authors: Ming Song, Xiaohong Li, Xiang Zhang, Hongxue Shi, Miriam B. Vos, Xiaoli Wei et al.
    American Journal of Physiology-Gastrointestinal and Liver Physiology

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