Rat Regulatory T Cell Multi-Color Flow Kit

For the flow cytometric analysis of rat regulatory T cells

Why is it Important to Identify Regulatory T Cells Using Multiple Established Markers?

Regulatory T cells (T_regs) are critical mediators of immune homeostasis. T_reg immunosuppressive effector functions act to prevent hyperimmunity, and reduced T_reg populations or T_reg activity are associated with autoimmune dysfunction. FoxP3, a defining intracellular marker of these cells, is a critical mediator of T_reg effector functions. The accurate identification of T_reg populations supports the advancement of autoimmune disease and organ transplantation research.  Using multiple established markers to identify T_reg cells increases confidence in cell identity and minimizes experimental variation. In addition, simultaneous use of multiple markers cost-effectively minimizes the required sample volume, which is critical in T_reg research where experiments are costly and sample volumes may be limited.

The Rat Regulatory T Cell Multi-Color Flow Cytometry Kit offers users an efficient and quantitative method to identify rat regulatory T cells by flow cytometry using three established cell lineage markers.

The Rat Regulatory T Cell Multi-Color Flow Cytometry Kit:

• Efficiently identifies rat T_reg cells using three established cell lineage markers.
• Minimizes experimental variability via simultaneous staining of multiple markers.
• Identifies multiple markers in a small sample volume.
• Does not require the use of secondary antibodies.
• Includes enough reagents to perform 50 assays.

The Rat Regulatory T Cell Multi-Color Flow Cytometry Kit includes three fluorochrome-conjugated primary antibodies, isotype controls, and buffers to fix, permeabilize, and wash cells.

• CD25-PE (goat IgG)
• CD4-FITC (Clone OX-38; mouse IgG_2A)
• FoxP3-APC (Goat IgG)
• Goat IgG-APC Isotype Control
• Flow Cytometry FoxP3 Staining Buffer (with 1% formaldehyde and 0.09% sodium azide)
• Flow Cytometry Staining Buffer (with BSA and 0.09% sodium azide)
• Includes enough reagents to perform 50 assays

Formaldehyde is a suspected carcinogen. Avoid contact with skin, eyes, and mucous membranes, and avoid inhaling fumes. In case of contact, wash immediately with water and seek medical advice.

Sodium azide may react with lead and copper plumbing to form explosive metallic azides. Flush with large volumes of water during disposal.

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Detection of CD4+ FoxP3+ Rat Regulatory T Cells by Multi-Color Flow Cytometry. Rat splenocytes were stained using reagents included in the Rat Regulatory T Cell Multi-Color Flow Cytometry Kit (Catalog # FMC015). Cells were stained simultaneously for the expression of rat regulatory T cell markers including CD25, CD4, and FoxP3. Quadrants were set based on isotype controls. The upper right quandrant shows FoxP3+ and CD4+ splenocyte populations.

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Detection of CD25+ FoxP3+ Rat Regulatory T Cells by Multi-Color Flow Cytometry. Rat splenocytes were stained using reagents included in the Rat Regulatory T Cell Multi-Color Flow Cytometry Kit (Catalog # FMC015). Cells were stained simultaneously for the expression of rat regulatory T cell markers including CD25, CD4, and FoxP3. Quadrants were set based on isotype controls. The upper right quandrant shows FoxP3+ and CD25+ splenocyte populations.