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Rat RTN1-A/NSP Antibody

Catalog #: MAB5996 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5996
MAB5996-SP
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Rat RTN1-A/NSP Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat RTN1-A/NSP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat RTN-A2B, recombinant human (rh) RTN1-A, and rhRTN3-A1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 559706
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant rat RTN1-A/NSP
Met73-Ser502
Accession # Q64548
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
Perfusion fixed frozen sections of rat brain (cerebellum)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RTN1-A/NSP

Reticulon-1 (RTN1-A/RTN1) is a 135 kDa multi-pass membrane protein localized to the endoplasmic reticulum of neurons. RTN1-A belongs to no known family of proteins. Rat RTN1-A is 777 amino acids (aa) in length and contains two transmembrane segments and a Reticulon domain (aa 592-759). There are also multiple potential sites for phosphorylation. Alternative splicing produces a second isoform known as RTN1-S, which is missing residues corresponding to aa 1-569 in RTN1-A, and has a 20 aa substitution for residues corresponding to aa 570-589. Rat RTN1-A is 82% aa identical to human RTN1-A

Long Name
Reticulon 1A/Neuroendocrine-specific Protein
Entrez Gene IDs
6252 (Human); 116644 (Rat)
Alternate Names
NSP; NSP-A; RTN1; RTN1A; RTN1-A

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