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Rat TIM-1/KIM-1/HAVCR Antibody

Catalog #: AF3689
36 Citations Datasheet / COA / SDS
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AF3689
AF3689-SP
TIM‑1/KIM‑1/HAVCR in Rat Kidney.
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Citations (36)
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Rat TIM-1/KIM-1/HAVCR Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat TIM-1/KIM-1/HAVCR in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) TIM-1 is observed, and less than 1% cross-reactivity with recombinant human TIM-1, recombinant rat TIM-2, rmTIM-3, rmTIM-4, rmTIM-6, and rmTIM-7 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat TIM-1/KIM-1/HAVCR
Ser18-Val238
Accession # O54947
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat TIM‑1/KIM‑1/HAVCR (Catalog # 3689-TM)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry TIM-1/KIM-1/HAVCR antibody in Rat Kidney by Immunohistochemistry (IHC-Fr). View Larger

TIM‑1/KIM‑1/HAVCR in Rat Kidney. TIM-1/KIM-1/HAVCR was detected in perfusion fixed frozen sections of rat kidney using 15 µg/mL Rat TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3689) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunocytochemistry/ Immunofluorescence Detection of Rat TIM-1/KIM-1/HAVCR by Immunocytochemistry/Immunofluorescence View Larger

Detection of Rat TIM-1/KIM-1/HAVCR by Immunocytochemistry/Immunofluorescence Immunohistochemical analysis of kidneys in LETO and OLETF rats.Representative immunohistochemical images of glomerular, tubular and tubulointerstitial lesions in each group, 12 weeks after starting to feed a Paigen diet. Scale bar, 100 μm. KIM-1, kidney injury molecule-1; MRP8, myeloid-related protein 8; NOX2, NADPH oxidase 2; OPN, osteopontin; TLR4, toll-like receptor 4; TNF-alpha, tumor necrosis factor-alpha. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0143979), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-1/KIM-1/HAVCR

KIM-1 (Kidney-injury molecule-1; also TIM-1 and HAVCR) is a 50-80 kDa, variably glycosylated, type I transmembrane glycoprotein that is a member of the TIM family of immunoglobulin superfamily molecules. This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM/KIM genes (# 1-8) vs. only three genes in human (# 1, 3, 4). It is unknown if the rat genome exactly parallels that of mouse. Rat KIM-1 is synthesized as a 307 amino acid (aa) precursor that contains a 21 aa signal sequence, a 214 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 51 aa cytoplasmic domain. The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple Thr-Ser-Pro motifs. The mucin region may undergo extensive O-linked glycosylation. The mouse KIM-1 gene is highly polymorphic and this may be reflected in rat. In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing an 85 kDa soluble molecule. A similar process has now been described in rat. The ECD of rat KIM-1 is 50% and 81% aa identical to human and mouse KIM-1 ECD, respectively. The only two reported ligands for KIM-1 are TIM-4 and the hepatitis A virus. However, others are believed to exist, and based on the ligand for TIM-3, one might be an S-type lectin. KIM-1 is found on CD4+ T cells and proximal renal tubular cells. KIM-1 ligation induces T cell proliferation and promotes cytokine production. In particular, it induces IL-4 production, and requires the KIM-1 cytoplasmic tyrosine phosphorylation motif. Alternatively, KIM-1 activation of TIM-4 induces cell cycle arrest.

Long Name
T Cell Immunoglobulin Mucin-1
Entrez Gene IDs
26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
Alternate Names
CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1

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Product Specific Notices

This product is covered by one or more of the following US Patents 7,300,652; 7,041,290; 6,664,385 and other US and foreign patents pending or issued.

Citations for Rat TIM-1/KIM-1/HAVCR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. An Immunohistochemical Investigation of Renal Phospholipidosis and Toxicity in Rats
    Authors: Jing Ying Ma, Sandra Snook, Sheryl Garrovillo, Charles Johnson, David La
    International Journal of Toxicology
  2. Assessment standards: comparing histopathology, digital image analysis, and stereology for early detection of experimental cisplatin-induced kidney injury in rats
    Authors: Katherine Shea, Sharron Stewart, Rodney Rouse
    Toxicologic Pathology
  3. Multisystem involvement, defective lysosomes and impaired autophagy in a novel rat model of nephropathic cystinosis
    Authors: Patrick Krohn, Laura Rita Rega, Marianne Harvent, Beatrice Paola Festa, Anna Taranta, Alessandro Luciani et al.
    Human Molecular Genetics
  4. Dual antiplatelet and anticoagulant APAC prevents experimental ischemia–reperfusion-induced acute kidney injury
    Authors: Raimo Tuuminen, Annukka Jouppila, Dan Salvail, Charles-E. Laurent, Marie-Claude Benoit, Simo Syrjälä et al.
    Clinical and Experimental Nephrology
  5. Kidney Injury Molecule-1 Is Specifically Expressed in Cystically-Transformed Proximal Tubules of the PKD/Mhm (cy/+) Rat Model of Polycystic Kidney Disease
    Authors: Stefan Gauer, Anja Urbschat, Norbert Gretz, Sigrid C. Hoffmann, Bettina Kränzlin, Helmut Geiger et al.
    International Journal of Molecular Sciences
  6. Conversion to Sirolimus Ameliorates Cyclosporine-Induced Nephropathy in the Rat: Focus on Serum, Urine, Gene, and Protein Renal Expression Biomarkers
    Authors: José Sereno, Sara Nunes, Paulo Rodrigues-Santos, Helena Vala, Petronila Rocha-Pereira, João Fernandes et al.
    BioMed Research International
  7. Kidney Injury Molecule-1 Enhances Endocytosis of Albumin in Renal Proximal Tubular Cells
    Authors: Xueying Zhao, Chen Jiang, Rebecca Olufade, Dong Liu, Nerimiah Emmett
    Journal of Cellular Physiology
  8. Tubule-Derived Follistatin Is Increased in the Urine of Rats with Renal Ischemia and Reflects the Severity of Acute Tubular Damage
    Authors: I Nagayama, K Takayanagi, H Hasegawa, A Maeshima
    Cells, 2023-03-04;12(5):.
  9. Effects of Mineralocorticoid Receptor Blockade and Statins on Kidney Injury Marker 1 (KIM-1) in Female Rats Receiving L-NAME and Angiotensin II
    Authors: J Huang, E Caliskan G, SK Gholami, KL Gawelek, RN Mitchell, LH Pojoga, JR Romero, GH Williams, GK Adler
    International Journal of Molecular Sciences, 2023-03-30;24(7):.
  10. Accelerated lysine metabolism conveys kidney protection in salt-sensitive hypertension
    Authors: MM Rinschen, O Palygin, A El-Meanawy, X Domingo-Al, A Palermo, LV Dissanayak, D Golosova, MA Schafroth, C Guijas, F Demir, J Jaegers, ML Gliozzi, J Xue, M Hoehne, T Benzing, BP Kok, E Saez, M Bleich, N Himmerkus, OA Weisz, BF Cravatt, M Krüger, HP Benton, G Siuzdak, A Staruschen
    Nature Communications, 2022-07-14;13(1):4099.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  11. Nephrotoxicity evaluation and proteomic analysis in kidneys of rats exposed to thioacetamide
    Authors: JY Lim, WW Jung, W Kim, KS Moon, D Sul
    Scientific Reports, 2022-04-27;12(1):6837.
    Species: Rat
    Sample Types: Tissue Lysates
    Applications: Western Blot
  12. Using Green Biosynthesized Lycopene-Coated Selenium Nanoparticles to Rescue Renal Damage in Glycerol-Induced Acute Kidney Injury in Rats
    Authors: A Al-Brakati, KF Alsharif, KJ Alzahrani, S Kabrah, O Al-Amer, AA Oyouni, OA Habotta, MS Lokman, AA Bauomy, RB Kassab, AE Abdel Mone
    International Journal of Nanomedicine, 2021-06-29;16(0):4335-4349.
    Species: Rat
    Sample Types: Plasma
    Applications: ELISA Capture
  13. Empagliflozin attenuates acute kidney injury after myocardial infarction in diabetic rats
    Authors: A Kuno, Y Kimura, M Mizuno, H Oshima, T Sato, N Moniwa, M Tanaka, T Yano, M Tanno, T Miki, T Miura
    Sci Rep, 2020-04-29;10(1):7238.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  14. Distinct inactivated bacterial-based immune modulators vary in their therapeutic efficacies for treating disease based on the organ site of pathology
    Authors: S Kalyan, M Bazett, HP Sham, M Bosiljcic, B Luk, S Dhanji, AM Costa, SWY Wong, MG Netea, DW Mullins, H Gunn
    Sci Rep, 2020-04-03;10(1):5901.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  15. Methionine abrogates the renoprotective effect of a low-protein diet against diabetic kidney disease in obese rats with type 2 diabetes
    Authors: M Kitada, Y Ogura, I Monno, J Xu, D Koya
    Aging (Albany NY), 2020-03-06;12(5):4489-4505.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  16. Canagliflozin, an SGLT2 inhibitor, normalizes renal susceptibility to type 1 cardiorenal syndrome through reduction of renal oxidative stress in diabetic rats
    Authors: Y Kimura, A Kuno, M Tanno, T Sato, K Ohno, S Shibata, K Nakata, H Sugawara, K Abe, Y Igaki, T Yano, T Miki, T Miura
    J Diabetes Investig, 2019-02-25;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. The 6-hydroxychromanol derivative SUL-109 ameliorates renal injury after deep hypothermia and rewarming in rats
    Authors: PC Vogelaar, M Roorda, EL de Vrij, MC Houwertjes, M Goris, H Bouma, AC van der Gr, G Krenning, RH Henning
    Nephrol. Dial. Transplant., 2018-12-01;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  18. Wnt4 is significantly upregulated during the early phases of cisplatin-induced acute kidney injury
    Authors: YX He, TT Diao, SM Song, CC Wang, Y Wang, CL Zhou, YB Bai, SS Yu, X Mi, XY Yang, QJ Wei, B Li
    Sci Rep, 2018-07-12;8(1):10555.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  19. Osteopontin deficiency ameliorates Alport pathology by preventing tubular metabolic deficits
    Authors: W Ding, K Yousefi, S Goncalves, BJ Goldstein, AL Sabater, A Kloosterbo, P Ritter, G Lambert, AJ Mendez, LA Shehadeh
    JCI Insight, 2018-03-22;3(6):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  20. High fructose diet feeding accelerates diabetic nephropathy in Spontaneously Diabetic Torii (SDT) rats
    Authors: K Toyoda, Y Suzuki, K Muta, T Masuyama, K Kakimoto, A Kobayashi, T Shoda, S Sugai
    J Toxicol Sci, 2018-01-01;43(1):45-58.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  21. Combination therapy of exendin-4 and allogenic adipose-derived mesenchymal stem cell preserved renal function in a chronic kidney disease and sepsis syndrome setting in rats
    Authors: CH Chen, BC Cheng, KH Chen, PL Shao, PH Sung, HJ Chiang, CC Yang, KC Lin, CK Sun, JJ Sheu, HW Chang, MS Lee, HK Yip
    Oncotarget, 2017-10-10;8(59):100002-100020.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  22. Endocytosis of Albumin Induces Matrix Metalloproteinase-9 by Activating the ERK Signaling Pathway in Renal Tubule Epithelial Cells
    Authors: X Chen, A Cobbs, J George, A Chima, F Tuyishime, X Zhao
    Int J Mol Sci, 2017-08-12;18(8):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  23. Combined therapy with melatonin and exendin-4 effectively attenuated the deterioration of renal function in rat cardiorenal syndrome
    Authors: KH Chen, CH Chen, CG Wallace, YT Chen, CC Yang, PH Sung, HJ Chiang, YL Chen, S Chua, HK Yip, JT Cheng
    Am J Transl Res, 2017-02-15;9(2):214-229.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  24. An Atherogenic Paigen-Diet Aggravates Nephropathy in Type 2 Diabetic OLETF Rats.
    Authors: Nozako M, Koyama T, Nagano C, Sato M, Matsumoto S, Mitani K, Yasufuku R, Kohashi M, Yoshikawa T
    PLoS ONE, 2015-11-25;10(11):e0143979.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  25. Altered expression profile of renal alpha(1D)-adrenergic receptor in diabetes and its modulation by PPAR agonists.
    Authors: Zhao X, Zhang Y, Leander M, Li L, Wang G, Emmett N
    J Diabetes Res, 2014-03-17;2014(0):725634.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  26. The uremic toxin adsorbent AST-120 abrogates cardiorenal injury following myocardial infarction.
    Authors: Lekawanvijit S, Kumfu S, Wang B, Manabe M, Nishijima F, Kelly D, Krum H, Kompa A
    PLoS ONE, 2013-12-13;8(12):e83687.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  27. Urinary levels of regenerating islet-derived protein III beta and gelsolin differentiate gentamicin from cisplatin-induced acute kidney injury in rats.
    Authors: Ferreira L, Quiros Y, Sancho-Martinez SM, Garcia-Sanchez O, Raposo C, Lopez-Novoa JM, Gonzalez-Buitrago JM, Lopez-Hernandez FJ
    Kidney Int., 2010-10-27;79(5):518-28.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  28. Sub-nephrotoxic doses of gentamicin predispose animals to developing acute kidney injury and to excrete ganglioside M2 activator protein.
    Authors: Quiros Y, Ferreira L, Sancho-Martinez SM, Gonzalez-Buitrago JM, Lopez-Novoa JM, Lopez-Hernandez FJ
    Kidney Int., 2010-08-18;78(10):1006-15.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  29. Databases for technical aspects of immunohistochemistry-2021 update
    Authors: MOROKI T, MATSUO S, HATAKEYAMA H et al.
    Journal of Toxicologic Pathology
  30. A Soluble Guanylate Cyclase Activator Inhibits the Progression of Diabetic Nephropathy in the ZSF1 Rat
    Authors: Carine M Boustany-Kari, Paul C Harrison, Hongxing Chen, Kathleen A Lincoln, Hu Sheng Qian, Holly Clifford et al.
    Journal of Pharmacology and Experimental Therapeutics
  31. Intrarenal arterial administration of human umbilical cord-derived mesenchymal stem cells effectively preserved the residual renal function of diabetic kidney disease in rat
    Authors: Ya Yue, Jui-Ning Yeh, John Y. Chiang, Pei-Hsun Sung, Yi-Ling Chen, Fanna Liu et al.
    Stem Cell Research & Therapy
  32. Rat Mesenchymal Stromal Cell Sheets Suppress Renal Fibrosis via Microvascular Protection
    Authors: Aya Imafuku, Masatoshi Oka, Yoei Miyabe, Sachiko Sekiya, Kosaku Nitta, Tatsuya Shimizu
    Stem Cells Translational Medicine
  33. Diabetes increases the susceptibility to acute kidney injury after myocardial infarction through augmented activation of renal Toll-like receptors in rats
    Authors: Kouhei Ohno, Atsushi Kuno, Hiromichi Murase, Shingo Muratsubaki, Takayuki Miki, Masaya Tanno et al.
    American Journal of Physiology-Heart and Circulatory Physiology
  34. Impaired endocytosis in proximal tubule from subchronic exposure to cadmium involves angiotensin II type 1 and cubilin receptors
    Authors: Mitzi Paola Santoyo-Sánchez, José Pedraza-Chaverri, Eduardo Molina-Jijón, Laura Arreola-Mendoza, Rafael Rodríguez-Muñoz, Olivier Christophe Barbier
    BMC Nephrology
  35. Therapeutic Effect of Curcumin on 5/6Nx Hypertriglyceridemia: Association with the Improvement of Renal Mitochondrial beta -Oxidation and Lipid Metabolism in Kidney and Liver
    Authors: Zeltzin Alejandra Ceja-Galicia, Fernando Enrique García-Arroyo, Omar Emiliano Aparicio-Trejo, Mohammed El-Hafidi, Guillermo Gonzaga-Sánchez, Juan Carlos León-Contreras et al.
    Antioxidants (Basel)
  36. Tissue Kim-1 and Urinary Clusterin as Early Indicators of Cisplatin-Induced Acute Kidney Injury in Rats
    Authors: Petra Vinken, Sofie Starckx, Erio Barale-Thomas, Adriana Looszova, Manisha Sonee, Nick Goeminne et al.
    Toxicologic Pathology

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