Rec. Human Linear Tetra-Ub Non-hydrolyzable Agarose, CF

Rec. Human Linear Tetra-Ub Non-hydrolyzable Agarose, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Tetra-Ubiquitin

With a predicted molecular weight of 34 kDa, Tetra-Ubiquitin chains are composed of four Ubiquitin monomers that are covalently linked through  isopeptide bonds, which typically form between a lysine residue of one Ubiquitin molecule and the C-terminal glycine residue of another Ubiquitin molecule (1). Each human Ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse Ubiquitin, respectively (2). Seven of the 76 aa in Ubiquitin are lysine residues that can participate in poly-Ubiquitin chain formation. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes (3-8).

This linear Ubiquitin fusion protein is resistant to the activity of deubiquitinating enzymes (DUB's) that cleave the peptide linkage between adjacent Ubiquitin molecules.  Ubiquitin is not expressed directly as free Ubiquitin, but rather as linear fusions either to itself or to certain ribosomal protein subunits.  These Ubiquitin-fusion precursors are proteolyzed by DUB's at the appropriate junction points to yield active Ubiquitin monomers with C-termini ending in GG.  There are likely several intracellular DUB's which perform this essential processing role.  This protein may be useful in analyzing interactions between linear Ubiquitin and proteins that contain Ubiquitin-associated domains (UBA's) or Ubiquitin-interacting motifs (UIM's). 

Related Research Areas

• Cell Structure and Signaling Molecules
• NFkB Pathway
• Parkinson's Disease Related Molecules
• Ubiquitin
• Wnt Intracellular Signaling