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Recombinant Bovine IFN-gamma Protein

Catalog #: 2300-BG
5 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
2300-BG-025/CF

With Carrier

Catalog # Availability Size / Price Qty
2300-BG-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (5)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Bovine IFN-gamma Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in an anti-viral assay using MDBK bovine kidney epithelial cells infected with vesicular stomatitis virus (VSV). Murkami, K. et al. (2001) Cytokine 13:18. The ED50 for this effect is 1.50-7.50 ng/mL.
Source
E. coli-derived bovine IFN-gamma protein
Gln24-Thr166 & Gln24-Arg162, both with an N-terminal Met
Accession #
NP_776511
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
17 kDa

Product Datasheets

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2300-BG (with carrier)

Product Datasheet
COA
SDS

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2300-BG/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2300-BG

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2300-BG/CF

Formulation Supplied as a 0.2 μm filtered solution in Acetonitrile and TFA.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature bovine IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 78%‑80% amino acid (aa) sequence identity with canine, feline, equine, and porcine IFN-gamma and 42%‑59% with cotton rat, human, mouse, rat, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

References
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Cerretti, D.P. et al. (1986) J. Immunol. 136:4561.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
Long Name
Interferon gamma
Entrez Gene IDs
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

Citations for Recombinant Bovine IFN-gamma Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Interferon-gamma modulates articular chondrocyte and osteoblast metabolism through protein kinase R-independent and dependent mechanisms
    Authors: SJ Gilbert, EJ Blain, DJ Mason
    Biochemistry and Biophysics Reports, 2022-09-07;32(0):101323.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Mucosal IFN&gamma production and potential role in protection in Escherichia coli O157:H7 vaccinated and challenged cattle
    Authors: RG Schaut, MV Palmer, PM Boggiatto, IT Kudva, CL Loving, VK Sharma
    Scientific Reports, 2021-05-07;11(1):9769.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Non-specific protection from respiratory tract infections in cattle generated by intranasal administration of an innate immune stimulant
    Authors: W Wheat, L Chow, V Rozo, J Herman, K Still Broo, A Colbath, R Hunter, S Dow
    PLoS ONE, 2020-06-25;15(6):e0235422.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Immunomodulatory and immunogenic properties of mesenchymal stem cells derived from bovine fetal bone marrow and adipose tissue
    Authors: O Huaman, J Bahamonde, B Cahuascanc, M Jervis, J Palomino, CG Torres, OA Peralta
    Res. Vet. Sci., 2019-03-21;124(0):212-222.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Immunoselected STRO-3(+) mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis
    Authors: A Abdalmula, LM Dooley, C Kaufman, EA Washington, JV House, BA Blacklaws, P Ghosh, S Itescu, SR Bailey, WG Kimpton
    Stem Cell Res Ther, 2017-02-07;8(1):22.
    Species: Ovine
    Sample Types: Plasma
    Applications: ELISA Developmet

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