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Recombinant Cotton Rat CXCL10/IP-10 Protein, CF

Catalog #: 1117-IP
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1117-IP-025
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Citations (2)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cotton Rat CXCL10/IP-10 Protein, CF Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CXCR3. The ED50 for this effect is 2-10 µg/mL.
Source
E. coli-derived cotton rat CXCL10/IP-10/CRG-2 protein
Ile22-Val94, with an N-terminal Met
Accession #
AAL16935
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
8.6 kDa

Product Datasheets

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1117-IP

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1117-IP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL10/IP-10/CRG-2

P-10, also named mob-1 in rat and crg-2 in mouse, is a member of the non-ELR-motif containing CXC chemokine subfamily and has been designated CXCL10. It is induced in various cell types (including keratinocytes, endothelial cells, fibroblasts, smooth muscle cells, monocytes/macrophages, astrocytes, neutrophils, hepatocytes, T cells, epithelial cells and mesothelial cells) in response to stimulation by type I and/or type II interferon as well as by LPS. IP-10 has been shown to chemoattract activated CD4+ and CD8+ T cells, activated NK cells and smooth muscle cells. It also induces the adhesion of activated T cells to VCAM and ICAM. In addition, IP-10 has been reported to antagonize the angiogenic activity of FGF. IP-10 binds to and activates CXCR3, the chemokine receptor that is shared by MIG/CXCL9 and I-TAC/CXCL11, two additional non-ELR CXC chemokines that are induced by interferon. Cotton rat IP-10 cDNA encodes a 94 amino acid (aa) residue precursor protein with a putative 21 aa signal peptide and a 73 aa mature protein with one potential N-linked glycosylation site. Mature cotton rat IP-10 shares 66%, 64%, and 66% aa sequence identity with rat, mouse and human IP10, respectively (1 - 7).

References
  1. Vanguri, P. and J.M. Farber (1990) J. Biol. Chem. 265:15049.
  2. Liang, P. et al. (1994) Proc. Natl. Acad. Sci. USA. 91:12515.
  3. Proost, P. et al. (1993) J. Immunol. 150:1000.
  4. Blanco, J.C. et al. (2001) GenBank Accession # AAL16959.
  5. Neville, L.F. (1997) Prog. Growth Factor Res. 8:207.
  6. Belperio, J.A. et al. (2000) J. Leukoc. Biol. 68:1.
  7. Soejima, K. and B.J. Rollins (2001) J. Immunol. 167:6576.
Entrez Gene IDs
3627 (Human); 15945 (Mouse)
Alternate Names
C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10

Citations for Recombinant Cotton Rat CXCL10/IP-10 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. T-Lymphocytes Traffic into the Brain across the Blood-CSF Barrier: Evidence Using a Reconstituted Choroid Plexus Epithelium.
    Authors: Nathalie Strazielle, Rita Creidy, Christophe Malcus, José Boucraut, Jean-François Ghersi-Eg
    PLoS ONE, 2016-03-04;0(0):1932-6203.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.
    Authors: Barkho BZ, Song H, Aimone JB
    Stem Cells Dev., 2006-06-01;15(3):407-21.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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