Recombinant Cynomolgus LIFR alpha His-tag Protein, CF Summary
Product Specifications
Gln45-Ser833, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10529-LR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Measured by its ability to inhibit LIF-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.7-7 μg/mL in the presence of 0.3 ng/mL of recombinant human LIF.
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2 μg/lane of Recombinant Cynomolgus LIFR alpha His-tag Protein (10529-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 113-150 kDa.
Reconstitution Calculator
Background: LIFR alpha
Leukemia Inhibitory Factor Receptor alpha (LIF R alpha ), also known as LIFR beta and CD118, is a 190 kDa type I transmembrane protein in the Interleukin-6 receptor family. Members of this family mediate the biological effects of Cardiotrophin-1, CLC, CNTF, IL-6, IL-11, IL-27, and Oncostatin M (1). Based on its similarity with human LIF R alpha, mature cynomolgus LIF R alpha is predicted to consist of a 789 amino acid (aa) extracellular domain (ECD) with two cytokine receptor homology domains, one WSxWS motif, and three fibronectin type III repeats, followed by a 26 aa transmembrane segment and a 238 aa cytoplasmic domain (2). Within the ECD, cynomolgus LIF R alpha shares 97% aa sequence identity with human LIF R alpha. LIF R alpha binds the pleiotropic cytokine LIF with low affinity (3). Binding affinity is increased by the ligand-induced association of LIF R alpha with the signal transducing subunit gp130 (4, 6). The LIF R alpha /gp130 receptor complex also transduces Oncostatin M signals, although LIF R alpha alone does not interact with Oncostatin M (4). gp130 associates with different ligand-specific receptors to form signaling receptor complexes for the other IL-6 family ligands (1). The CNTF receptor is a ternary complex that contains CNTF R alpha and gp130 as well as LIF R alpha (6, 7). LIF R alpha is widely expressed, and LIF induces the proliferation, differentiation, and activation of cells in many tissues (8, 9). In particular, LIF R alpha plays an important role in several aspects of early pregnancy such as blastocyst implantation in the uterus (10-12).
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- Gearing, D.P. et al. (1991) EMBO J. 10:2839.
- Layton, M.J. et al. (1992) Proc. Natl. Acad. Sci. 89:8616.
- Gearing, D.P. et al. (1992) Science 255:1434.
- Giese, B. et al. (2005) J. Cell Sci. 118:5129.
- Ip, N.Y. et al. (1992) Cell 69:1121.
- Davis, S. et al. (1993) Science 260:1805.
- Metcalf, D (2003) Stem Cells 21:5.
- Kubota, Y. et al. (2008) J. Clin. Invest. 118:2393.
- Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
- Stewart, C.L. et al. (1992) Nature 359:76.
- Cheng, J.-G. et al. (2001) Proc. Natl. Acad. Sci. 98:8680.
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