Recombinant Cynomolgus Monkey Nectin-2/CD112 His Protein, CF

Recombinant Cynomolgus Monkey Nectin-2/CD112 His Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Nectin-2/CD112

Nectins are a small family of Ca⁺⁺-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions (1). The nectin family has at least four members (nectin-1-4), all of which show alternate splicing (except for Nectin-4), a transmembrane (TM) region (except for Nectin-1 gamma ), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus (1). Nectin-2 is a type I TM glycoprotein that is found on a variety of cell types (2, 3). Cynomolgus Nectin-2 is synthesized as a 538 amino acid precursor. It contains a 31 amino acid (aa) signal sequence, a 329 aa extracellular domain (ECD), a 22 aa TM segment, and a 156 aa cytoplasmic domain. The ECD contains one N-terminal V-type Ig domain and two C2-type Ig domains. The V-domain is believed to mediate nectin binding to its ligands (4).  The ECD of cynomolgus Nectin-2 shows 96% aa sequence identity with the ECD of human Nectin-2. Nectin-2 is known to bind the pseudorabies virus, and herpes simplex virus-2 (HSV-2), but not HSV-1. It does not bind poliovirus. As a cell adhesion molecule, Nectin-2 will form cis-homodimers (same cell), followed by trans-dimers (across cells). Nectin-2 will not cis-dimerize with other nectins, but will cis-dimerize with its two splice forms. Notably, a Nectin-2 cis-dimer on one cell will heterodimerize with a Nectin-3 cis-dimer on another cell (1). Nectin-2 is found concentrated in adherens junctions, and exists on neurons, endothelial cells, epithelial cells and fibroblasts.

Related Research Areas

• Adhesion Molecules in Angiogenesis
• Adhesion Molecules on Endothelial Cells
• Adhesion Molecules on VSMC
• Cell to Cell Adhesion Disassembly During EMT
• Co-stimulatory and Co-inhibitory Molecules
• Endothelial Cell Markers
• Epithelial Cell Markers and Intracellular Molecules
• Epithelial Cell Polarity
• Immunoglobulin Superfamily CAMs
• Infectious Diseases
• Nectin and Nectin-like Binding Receptors and Ligands
• Nectins
• Neuronal Lineage Markers
• Search Immune Checkpoint Targets
• Surface Ectodermal Cells