Recombinant Cynomolgus PD-L2/B7-DC Fc Chimera Protein, CF
Recombinant Cynomolgus PD-L2/B7-DC Fc Chimera Protein, CF Summary
Product Specifications
| Cynomolgus Monkey PD-L2/B7-DC (Leu20-Pro219) Accession # XP_005581839 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9178-PL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) is coated at 1 µg/mL (100 μL/well), Recombinant Cynomolgus Monkey PD‑L2/B7‑DC Fc Chimera (Catalog # 9178-PL) binds with a typical ED50 of 0.1-0.6 μg/mL.
Background: PD-L2/B7-DC
Programmed Death Ligand 2 (PD-L2), also known as B7-DC and butyrophilin-like protein, is a transmembrane member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1). Within the ECD, cynomolgous PD-L2 shares 71% and 96% aa sequence identity with mouse and human PD-L2, respectively (2, 3). PD-L2 is expressed on dendritic cells, subsets of activated CD4+ and CD8+ T cells, and memory B cells that differentiate into plasma cells (3-5). At inflammatory sites such as rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is up-regulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells (6-10). PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand up-regulation while inhibiting IL-4 production (2, 3, 11, 12). In addition, PD-L2 binds to RGM-B on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance (13). In asthma, PD-L2 suppresses IL-5 and IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production (8, 10).
- Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
- Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
- Tseng, S.-Y. et al. (2001) J. Exp. Med. 193:839.
- Messal, N. et al. (2011) Mol. Immunol. 48:2214.
- Zuccarino-Catania, G.V. et al. (2014) Nat. Immunol. 15:631.
- Guo, G. et al. (2012) Clin. Rheumatol. 31:271.
- Loke, P. and J.P. Allison (2003) Proc. Natl. Acad. Sci. USA 100:5336.
- Matsumoto, K. et al. (2004) J. Immunol. 172:2530.
- Stanciu, L.A. et al. (2006) J. Infec. Dis. 193:404.
- Lewkowich, I.P. et al. (2013) Mucosal Immun. 6:728.
- Ghiotto, M. et al. (2010) Int. Immunol. 22:651.
- Shin, T. et al. (2003) J. Exp. Med. 198:31.
- Xiao, Y. et al. (2014) J. Exp. Med. 211:943.
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