Recombinant Cynomolgus/Rhesus EDAR His-tag Protein, CF Summary
Product Specifications
Glu27-Ala187, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
11350-ER
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
View Larger
Measured by its binding ability in a functional ELISA. When Recombinant Human EDA-A1/Ectodysplasin A1 Protein (3944-ED) is immobilized at 2.00 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque EDAR His-tag Protein (Catalog # 11350-ER) binds with an ED50 of 0.200-3.00 μg/mL.
View Larger
2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque EDAR His-tag Protein (Catalog # 11350-ER) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 28-41 kDa.
Reconstitution Calculator
Background: EDAR
EDAR is a type I transmembrane protein which is a member of the TNF Receptor Superfamily (TNFRSF). The extracellular domain contains 14 cysteine residues, six of which approximate the TNFRSF cysteine-rich region; the cytoplasmic domain contains a region with homology to the death domains found in other TNFRSF members. Based on its high homology with human EDAR, cynomologus EDAR is predicted to be a 488 amino acid (aa) protein with a 26 aa signal, a 163 aa extracellular domain, a 22 aa transmembrane domain, and a 277 aa cytoplasmic domain. The cynomolgus and human EDAR homologs share 99% identity. Within the TNFRSF, EDAR shares the highest homologies with XEDAR and TNFRSF19/TROY. EDA-A1 is the EDAR ligand. EDA and EDAR have been associated with hypohidrotic ectodermal dysplasia (HED). HED is characterized by abnormalities in hair, teeth and eccrine sweat gland morphogenesis. HED was initially found to associate with two gene loci, tabby and downless. Tabby was later identified as the gene for EDA and downless as the autosomal EDAR gene. EDA has two splice variants, EDA-A1 and EDA-A2, which differ by only two amino acids. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds EDAR, whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF member with high homology to EDAR. Mutations in EDA, EDAR and XEDAR have been associated with HED.
- Headon, D.J. and P.A. Overbeek (1999) Nat.Genet. 22:370.
- Kumar, A. et al. (2000) J. Biol. Chem. 276:2668.
- Monreal, A.W. et al. (1999) Nat. Genet. 22:366.
- Schneider, P. et al. (2001) J. Biol. Chem. 276:18819.
- Srivastava, A.K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:13069.
- Yan, M. et al. (2000) Science 290:523.
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Cynomolgus/Rhesus EDAR His-tag Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Cynomolgus/Rhesus EDAR His-tag Protein, CF and earn rewards!
Have you used Recombinant Cynomolgus/Rhesus EDAR His-tag Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
