Recombinant Cynomolgus/Rhesus NKG2D Fc Chimera Protein, CF

Catalog #: 10960-NK Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10960-NK-050

Recombinant Cynomolgus Monkey/Rhesus Macaque NKG2D/CD314 Fc Chimera Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus/Rhesus NKG2D Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey/Rhesus Macaque NKG2D/CD314 Fc Chimera (Catalog # 10960-NK) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human MICA Fc Chimera Avi-tag (Catalog # AVI1300) binds with an ED₅₀ of 25.0‑200 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived NKG2D/CD314 protein

MD	Human IgG₁ (Pro100-Lys330)	IEGR	Cynomologus Monkey NKG2D (Trp74-Val216) Accession # P61252.1
N-terminus			C-terminus

Accession #

P61252.1

N-terminal Sequence
Analysis

Met

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

43 kDa

SDS-PAGE

45-65 kDa, under reducing conditions.

Product Datasheets

10960-NK

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10960-NK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Cynomolgus Monkey/Rhesus Macaque NKG2D/CD314 Fc Chimera Protein (Catalog # 10960-NK) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human MICA Fc Chimera Avi-tag (AVI1300) binds with an ED₅₀ of 25.0-200 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque NKG2D/CD314 Fc Chimera Protein (Catalog # 10960-NK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-65 kDa and 90-130 kDa, respectively.

Reconstitution Calculator

Background: NKG2D/CD314

Natural killer group 2, member D (NKG2D) is a type II lectin-like transmembrane glycoprotein is an activating receptor involved in both innate and adaptive immunities. Cynomologus NKG2D consists of an extracellular domain (ECD) with a C-type lectin domain, a transmembrane segment and a short cytoplasmic domain lacking signaling properties. The ECD lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands (1). The ECD of cynomologus NKG2D shares 98% amino acid sequence identity with the ECD of human NKG2D. NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells and NKT cells and additionally occurs on macrophages in mouse (1 - 3). The NKG2D receptor consists of a homodimer and has been shown to mediate immune responses and determine the activation status of NK cells (4). In human, ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3 (3, 5). Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection (6, 7). Alternative splicing of the NKG2D results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal (7). NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection (6, 8).

References

Li, P. et al. (2001) Nature Immunol. 2:443.
Steinle, A. et al. (2001) Immunogenetics 53:279.
Wang, J. et al. (2020) Biomol. 10:301.
Garrity, D. et al. (2005) Proc. Natl. Acad. Sci. USA 102:7641.
Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.

Long Name

Natural Killer G2D

Entrez Gene IDs

22914 (Human); 27007 (Mouse); 102120479 (Cynomolgus Monkey)

Alternate Names

CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2D; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence