Recombinant Cynomolgus/Rhesus NKp30 Fc Chimera Protein, CF
Recombinant Cynomolgus/Rhesus NKp30 Fc Chimera Protein, CF Summary
Product Specifications
| Cynomolgus Monkey NKp30/NCR3 (Leu19-Gly137) Accession # XP_005553602.1 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10947-NK
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human B7-H6 His-tag Protein (9309-B7) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey/Rhesus Macaque NKp30/NCR3 Fc Chimera (Catalog # 10947-NK) that produces 50% of the optimal binding response is 4.00-24.0 ng/mL.
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2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque NKp30/NCR3 Fc Chimera (Catalog # 10947-NK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-65 kDa and 100-130 kDa, respectively.
Reconstitution Calculator
Background: NKp30/NCR3
NKp30, also termed Natural cytotoxicity receptor 3 (NCR3), along with NKp44 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors" (1). These receptors play a major role in triggering NK-mediated killing of most tumor cell lines. Mature NKp30 consists of an extracellular domain (ECD) with a V‑type Ig-like fold, a single transmembrane region and a short cytoplasmic domain (2). A charged residue in the transmembrane domain of NKp30 is responsible for association with the tyrosine-based activating motif (ITAM)‑bearing accessory protein CD3 zeta (2). The ECD of cynomologus NKp30 shares 93% amino acid sequence identity with the ECD of human NKp30. In human, several NKp30 isoforms arising from alternative splicing are known to exist (3). NKp30 is expressed on both resting and activated NK cells of the CD56dim, CD16+ subset and account for ~85% of NK cells found in peripheral blood and spleen (4). While NKp30 is absent from the CD56bright, CD16- subset that constitutes the majority of NK cells in lymph node and tonsil, its expression is up-regulated in these cells upon IL-2 activation (4). Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK-mediated lysis of immature dendritic cells (2, 5). The B7 family member B7-H6, a tumor cell ligand, has been identified as activating NKp30 (6). Additionally, soluble galectin-3 released from tumor cells was found to bind NKp30 thereby inhibiting NKp30-mediated cytotoxicity (7).
- Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
- Pende, D. et al. (1999) J. Exp. Med. 190:1505.
- Pinheiro, P.H. et al. (2020) Br J Pharmacol 177:4563.
- Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
- Ferlazzo, G. et al. (2002) J. Exp. Med. 195:343.
- Brandt, C.S. et al. (2009) J Exp Med. 206:1495.
- Kellner, C. et al. (2012) J Immunol. 189:5037.
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