Recombinant EBOV GP Protein, CF
Recombinant EBOV GP Protein, CF Summary
Product Specifications
| Z. ebolavirus GP1 (Ile33-Arg501) Accession # Q05320 | |
| Z. ebolavirus
GP2 (Glu502-Gln650) Accession # Q05320 | HHHHHH |
| N-terminus | C-terminus |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9016-EB
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution | Reconstitute at 500 μg/mL in water. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: EBOV GP
The GP glycoprotein encoded by the genome of Ebola family viruses is a critical molecule for the pathogenicity of Ebolavirus hemorrhagic viruses (1, 2). It is processed into distinct forms for virus capsule or cell surface presentation or release from virus infected cells. The GP precursor protein is cleaved by furin at a multibasic site to yield a 140 kDa N-terminal fragment (GP1) and a 26 kDa C-terminal fragment (GP2) which remain disulfide linked (3). GP1 is entirely extracellular while GP2 is a transmembrane protein (4). Heterodimers of GP1-GP2 can further associate into trimers (5). GP expressed on virus infected cells can be shed by TACE mediated cleavage, liberating a disulfide linked complex of soluble GP1 and truncated GP2 (4-6). GP binds to multiple C-type lectins on target cell surfaces, including CLEC10A/MGL, DC-SIGN, and DC-SIGNR (7-9). Following internalization, GP1 is cleaved by Cathepsin B and Cathepsin L and then interacts with Niemann-Pick C1 (NPC1) in the endosomal membrane (10-12).
- Yang, Z.-Y. et al. (2000) Nat. Med. 6:886.
- de La Vega, M.-A. et al. (2015) Viral Immunol. 28:3.
- Volchkov, V.E. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5762.
- Volchkov, V.E. et al. (1998) Virology 245:110.
- Sanchez, A. et al. (1998) J. Virol. 72:6442.
- Dolnik, O. et al. (2004) EMBO J. 23:2175.
- Takada, A. et al. (2004) J. Virol. 78:2943.
- Alvarez, C.P. et al. (2002) J. Virol. 76:6841.
- Simmons, G. et al. (2003) Virology 305:115.
- Schornberg, K. et al. (2006) J. Virol. 80:4174.
- Chandran, K. et al. (2005) Science 308:1643.
- Cote, M. et al. (2011) Nature 477:344.
Citations for Recombinant EBOV GP Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Systems approach to define humoral correlates of immunity to Shigella
Authors: B Bernshtein, E Ndungo, D Cizmeci, P Xu, P Ková?, M Kelly, D Islam, ET Ryan, KL Kotloff, MF Pasetti, G Alter
Cell Reports, 2022-08-16;40(7):111216.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Control -
Robust IgM responses following intravenous vaccination with Bacille Calmette-Gu�rin associate with prevention of Mycobacterium tuberculosis infection in macaques
Authors: EB Irvine, A O'Neil, PA Darrah, S Shin, A Choudhary, W Li, W Honnen, S Mehra, D Kaushal, HP Gideon, JL Flynn, M Roederer, RA Seder, A Pinter, S Fortune, G Alter
Nature Immunology, 2021-11-22;0(0):.
Species: Rhesus Macaque
Sample Types: In Vivo
Applications: In Vivo Control -
Serology and Behavioral Perspectives on Ebola Virus Disease Among Bushmeat Vendors in Equateur, Democratic Republic of the Congo, After the 2018 Outbreak
Authors: A Lucas, C Kumakamba, CE Lange, E Obel, G Miningue, J Likofata, A Gillis, M LeBreton, DJ McIver, J Euren, C Kreuder Jo, T Goldstein, JJ Muyembe-Ta, K Saylors
Open Forum Infect Dis, 2020-07-17;7(8):ofaa295.
Species: Human
Sample Types: Serum
Applications: ELISA Capture
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