Recombinant Equine IL-4 Protein Summary
Product Specifications
Lys26-Cys137
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1809-EL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1809-EL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: IL-4
Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa ‑ 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1-3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Equine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature equine IL-4 shares 53% - 60% aa sequence identity with bovine, goat, human, ovine, and porcine
IL-4 and 38%-40% aa sequence identity with mouse and rat IL-4. IL-4 exerts its effects through two receptor complexes (5, 6). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and
-21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2‑biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (7-10). IL-4 plays a dominant role in the development of allergic inflammation and asthma (9, 11).
- Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
- Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
- Vandergrifft, E.V. et al. (1994) Vet. Immunol. Immunopathol. 40:379.
- Redfield, C. et al. (1991) Biochemistry 30:11029.
- Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
- Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
- Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
- Corthay, A. (2006) Scand. J. Immunol. 64:93.
- Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
- Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
- Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Citations for Recombinant Equine IL-4 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
3
Citations: Showing 1 - 3
Filter your results:
Filter by:
-
Equine Arteritis Virus in Monocytic Cells Suppresses Differentiation and Function of Dendritic Cells
Authors: NA Moyo, D Westcott, R Simmonds, F Steinbach
Viruses, 2023-01-16;15(1):.
Species: Equine
Sample Types: Whole Cells
Applications: Bioassay -
Effects of priming with cytokines on intracellular survival and replication of Rhodococcus equi in equine macrophages
Authors: LJ Berghaus, S Giguère, AI Bordin, ND Cohen
Cytokine, 2017-12-12;102(0):7-11.
Species: Equine
Sample Types: Whole Cells
Applications: Bioassay -
IL-4 stimulates the expression of CXCL-8, E-selectin, VEGF, and inducible nitric oxide synthase mRNA by equine pulmonary artery endothelial cells.
Authors: Huang H, Lavoie-Lamoureux A, Moran K, Lavoie JP
Am. J. Physiol. Lung Cell Mol. Physiol., 2007-05-01;292(5):L1147-54.
Species: Equine
Sample Types: Whole Cells
Applications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Equine IL-4 Protein
There are currently no reviews for this product. Be the first to review Recombinant Equine IL-4 Protein and earn rewards!
Have you used Recombinant Equine IL-4 Protein?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
