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Recombinant Feline GM-CSF Protein

Catalog #: 987-FL
2 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
987-FL-010/CF

With Carrier

Catalog # Availability Size / Price Qty
987-FL-010
R&D Systems Recombinant Proteins and Enzymes
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Citations (2)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Feline GM-CSF Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 2‑8 ng/mL.
Source
E. coli-derived feline GM-CSF protein
Ala18-Lys144 (Met36Ile, Thr56Ala & Lys126Asn), with an N-terminal Met
Accession #
AAC06041
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
14.6 kDa

Product Datasheets

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987-FL (with carrier)

Product Datasheet
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SDS

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987-FL/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

987-FL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

987-FL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GM-CSF

GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3 - 5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha -helices (8 - 10). Mature feline GM-CSF shares 52% - 56% amino acid sequence identity with mouse and rat GM-CSF and 67% - 72% canine, human, and porcine GM-CSF. GM-CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta  chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha (13). Feline and human GM-CSF show cross-species activity (14, 15).

References
  1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653. 
  2. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509. 
  3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163. 
  4. Cao, Y. (2007) J. Clin. Invest. 117:2362. 
  5. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405. 
  6. Heuser, M. et al. (2007) Semin. Hematol. 44:148. 
  7. Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321. 
  8. Kaushansky, K. et al. (1992) Biochemistry 31:1881.
  9. Diederichs, K. et al. (1991) Science 254:1779.
  10. Dunham, S.P. and J. Bruce (2004) Gene 332:97.
  11. Onetto-Pothier, N. et al. (1990) Blood 75:59.
  12. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.
  13. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
  14. Sprague, W.S. et al. (2005) J. Comp. Pathol. 133:136.
  15. Dunham, S.P. and J. Bruce (2004) Gene 332:97.
Long Name
Granulocyte Macrophage Growth Factor
Entrez Gene IDs
1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
Alternate Names
colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; CSF-2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; Molgramostim; molgramostin; Sargramostim

Citations for Recombinant Feline GM-CSF Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Altered bone marrow dendritic cell cytokine production to toll-like receptor and CD40 ligation during chronic feline immunodeficiency virus infection.
    Authors: Lehman TL, O'Halloran KP, Fallon SA, Habermann LM, Campbell JA, Nordone S, Dean GA, Hoover EA, Avery PR
    Immunology, 2008-09-02;126(3):405-12.
    Species: Feline
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Generation of feline dendritic cells derived from peripheral blood monocytes for in vivo use.
    Authors: Freer G, Matteucci D, Mazzetti P, Bozzacco L, Bendinelli M
    Clin. Diagn. Lab. Immunol., 2005-10-01;12(10):1202-8.
    Species: Feline
    Sample Types: Whole Cells
    Applications: Bioassay

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