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Recombinant HIV-1 Gag p24 His-tag Protein, CF

Catalog #: 11243-HV Datasheet / COA / SDS
E. coli-expressed
Catalog # Availability Size / Price Qty
11243-HV-100
Recombinant HIV-1 Gag p24 His-tag Protein SDS-PAGE.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant HIV-1 Gag p24 His-tag Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Activity
Bioassay data are not available.
Source
E. coli-derived viral HIV-1 Gag p24 protein
Pro149-Gly354, with a C-terminal 6-His tag and a N-terminal Met
Accession #
NP_057850.1
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
24 kDa
SDS-PAGE
21-25 kDa, under reducing conditions.

Product Datasheets

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11243-HV

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11243-HV

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, Urea with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE View Larger

2 μg/lane of Recombinant HIV-1 Gag p24 His-tag Protein (Catalog # 11243-HV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 21-25 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background:

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load. This product is intended to be used to generate standard curves and/or positive controls for use in HIV-1 Gag p24 immunoassays.

FAQs

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