Recombinant Human ACE-2 Fc Chimera Protein, CF
Recombinant Human ACE-2 Fc Chimera Protein, CF Summary
Product Specifications
| Human ACE-2 (Gln18-Ser740) Accession # Q9BYF1.2 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10544-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, ZnCl2 and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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2 μg/lane of Recombinant Human ACE-2 Fc Chimera (10544-ZN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ~126 kDa under reducing conditions.
Reconstitution Calculator
Background: ACE-2
Angiotensin I Converting Enzyme (ACE-2), also called ACEH (ACE homologue), is a dimeric, zinc-dependent metalloprotease of the ACE family that also includes somatic and germinal ACE (1, 2). ACE-2 mRNA is found at high levels in heart, testis, and kidney and at lower levels in a wide variety of tissues (1, 3). ACE-2 is the SARS-CoV and SARS-CoV2 Spike protein receptor in vivo (4-6), functions catalytically as a carboxypeptidase to cleave several substrates including angiotensins I and II, and acts as a partner for B0AT1-family amino acid transporters (1, 2). Through these functions, ACE-2 has been shown to be involved in several diseases including SARS, COVID19, acute lung injury (4, 7), heart disease (8), liver and lung fibrosis (9), inflammatory lung disease (10), and cardiopulmonary disease (11). Full length ACE-2 protein includes an extracellular region composed of a single N-terminal peptidase domain and C-terminal collectrin-like domain (CLD), a transmembrane domain, and a short cytoplasmic tail (12). The N-terminal peptidase region is required for binding to SARS-CoV and SARSCoV2 spike proteins, while the CLD contains a region that promotes dimerization and association with amino acid transporters (2). The peptidase domain contains a long deep cleft that undergoes a large hinge-bending movement at substrate and inhibitor binding (12). Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity and inhibitors of ACE-2 do not inhibit ACE activity (13).
- Kuba, K. et al. (2010) Pharmacol. Ther. 128:119.
- Yan, et al. (2020) Science 367:1444.
- Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
- Kuba, K. et al. (2005) Nature Med. 11:875.
- Hoffman, M. et al. (2020) Cell.181:1.
- Wrapp, et al. (2020) Science 367:1260.
- Imai, Y. et al. (2005) Nature 436:112.
- Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
- Schrom, E. et al. (2017) Mol. Therapy Nuc. Acid 7:350.
- Jia, H. et al. (2016) Shock. 46:239.
- Cole-Jeffrey, C.T. et al. (2015) J. Cadiovasc. Pharmacol. 66:540.
- Towler, P. et al. (2004) J. Biol. Chem. 279:17996.
- Crackower, M.A. et al. (2002) Nature 417:822.
Citations for Recombinant Human ACE-2 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Decoy peptides effectively inhibit the binding of SARS-CoV-2 to ACE2 on oral epithelial cells
Authors: Loi, LK;Yang, CC;Lin, YC;Su, YF;Juan, YC;Chen, YH;Chang, HC;
Heliyon
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Capture -
Omicron subvariant BA.5 efficiently infects lung cells
Authors: Hoffmann, M;Wong, LR;Arora, P;Zhang, L;Rocha, C;Odle, A;Nehlmeier, I;Kempf, A;Richter, A;Halwe, NJ;Schön, J;Ulrich, L;Hoffmann, D;Beer, M;Drosten, C;Perlman, S;Pöhlmann, S;
Nature communications
Species: Human
Sample Types: Transfected Whole Cells
Applications: Bioassay
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