Recombinant Human ACE-2 His-tag Avi-tag Protein (CHO), CF

Catalog #: AVI10579 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI10579-020

Recombinant Human ACE-2 His-tag Avi-tag Protein (CHO) Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human ACE-2 His-tag Avi-tag Protein (CHO), CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant SARS-CoV-2 Spike RBD Fc Chimera (Catalog # 10499-CV) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human ACE-2 His-tag Avi-tag (CHO Expressed) (Catalog # AVI10579) binds with an ED₅₀ of 1.2-9.6 ng/mL. Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007). The specific activity is >700 pmol/min/μg, as measured under the described conditions.

Source

Chinese Hamster Ovary cell line, CHO-derived human ACE-2 protein

Human ACE-2 (Gln18 - Ser740) Accession # Q9BYF1.2	10-His tag	Avi-tag
N-terminus		C-terminus

Accession #

Q9BYF1.2

N-terminal Sequence
Analysis

Protein identity confirmed by mass spectrometry.

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

87 kDa

SDS-PAGE

103-113 kDa, under reducing conditions

Product Datasheets

AVI10579

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10579

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl, ZnCl₂ and Glycerol.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Binding Activity

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When Recombinant SARS-CoV-2 Spike RBD Fc Chimera (10499-CV) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human ACE-2 His-tag Avi-tag (CHO Expressed) (Catalog # AVI10579) binds with an ED₅₀ of 1.2-9.6 ng/mL.

Enzyme Activity

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Biotinylated Recombinant Human ACE‑2 His-tag Avi-tag (CHO Expressed) (Catalog # AVI10579) is measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (ES007).

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human ACE-2 His-tag Avi-tag Protein (Catalog # AVI10579) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ~109 kDa under reducing conditions.

Reconstitution Calculator

Background: ACE-2

Angiotensin I Converting Enzyme (ACE-2), also called ACEH (ACE homologue), is a dimeric, zinc-dependent metalloprotease of the ACE family that also includes somatic and germinal ACE (1, 2). ACE-2 mRNA is found at high levels in heart, testis, and kidney and at lower levels in a wide variety of tissues (1, 3). ACE-2 is the SARS-CoV and SARS-CoV2 Spike protein receptor in vivo (4-6), functions catalytically as a carboxypeptidase to cleave several substrates including angiotensins I and II, and acts as a partner for B0AT1-family amino acid transporters (1, 2). Through these functions, ACE-2 has been shown to be involved in several diseases including SARS, COVID19, acute lung injury (4, 7), heart disease (8), liver and lung fibrosis (9), inflammatory lung disease (10), and cardiopulmonary disease (11). Full length ACE-2 protein includes an extracellular region composed of a single N-terminal peptidase domain and C-terminal collectrin-like domain (CLD), a transmembrane domain, and a short cytoplasmic tail (12). The N-terminal peptidase region is required for binding to SARS-CoV and SARSCoV2 spike proteins, while the CLD contains a region that promotes dimerization and association with amino acid transporters (2). The peptidase domain contains a long deep cleft that undergoes a large hinge-bending movement at substrate and inhibitor binding (12). Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity and inhibitors of ACE-2 do not inhibit ACE activity (13).

References

Kuba, K. et al. (2010) Pharmacol. Ther. 128:119.
Yan, R. et al. (2020) Science 367:1444.
Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
Kuba, K. et al. (2005) Nature Med. 11:875.
Hoffmann, M. et al. (2020) Cell.181:1.
Wrapp, D. et al. (2020) Science 367:1260.
Imai, Y. et al. (2005) Nature 436:112.
Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
Schrom, E. et al. (2017) Mol. Therapy Nuc. Acid 7:350.
Jia, H. et al. (2016) Shock. 46:239.
Cole-Jeffrey, C.T. et al. (2015) J. Cardiovasc. Pharmacol. 66:540.
Towler, P. et al. (2004) J. Biol. Chem. 279:17996.
Crackower, M.A. et al. (2002) Nature 417:822.

Long Name

Angiotensin I Converting Enzyme 2

Entrez Gene IDs

59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)

Alternate Names

ACE2; ACE-2; ACEH; ACEHangiotensin I converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; DKFZp434A014; EC 3.4.17; EC 3.4.17.23; Metalloprotease MPROT15