Angiotensin I Converting Enzyme (ACE-2), also called ACEH (ACE homologue), is a dimeric, zinc-dependent metalloprotease of the ACE family that also includes somatic and germinal ACE (1, 2). ACE-2 mRNA is found at high levels in heart, testis, and kidney and at lower levels in a wide variety of tissues (1, 3). ACE-2 is the SARS-CoV and SARS-CoV2 Spike protein receptor in vivo (4-6), functions catalytically as a carboxypeptidase to cleave several substrates including angiotensins I and II, and acts as a partner for B0AT1-family amino acid transporters (1, 2). Through these functions, ACE-2 has been shown to be involved in several diseases including SARS, COVID19, acute lung injury (4, 7), heart disease (8), liver and lung fibrosis (9), inflammatory lung disease (10), and cardiopulmonary disease (11). Full length ACE-2 protein includes an extracellular region composed of a single N-terminal peptidase domain and C-terminal collectrin-like domain (CLD), a transmembrane domain, and a short cytoplasmic tail (12). The N-terminal peptidase region is required for binding to SARS-CoV and SARSCoV2 spike proteins, while the CLD contains a region that promotes dimerization and association with amino acid transporters (2). The peptidase domain contains a long deep cleft that undergoes a large hinge-bending movement at substrate and inhibitor binding (12). Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity and inhibitors of ACE-2 do not inhibit ACE activity (13).
Recombinant Human ACE-2 Protein, CF
R&D Systems | Catalog # 933-ZN
Key Product Details
- R&D Systems NS0-derived Recombinant Human ACE-2 Protein (933-ZN)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Structure / Form
Applications
Why choose R&D Systems ACE-2 Protein?
- Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 90%.
- Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
- Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
- Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.
Find the Fc chimera ACE-2 protein here ACE-2 Fc Chimera
Other ACE-2 species available ACE-2 Proteins
Product Specifications
Source
Gln18-Ser740, with a C-terminal 10-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Measured by its binding ability in a functional ELISA.
When
Recombinant SARS-CoV-2 Spike RBD Fc Chimera (Catalog #
10499-CV) is immobilized
at 0.2 µg/mL (100 µL/well), Recombinant Human ACE-2 (Catalog # 933-ZN) binds
with an ED50 of 1.00-12.0 ng/mL.
Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007).
The specific activity is >800 pmol/min/µg, as measured under the described conditions.
Reviewed Applications
Read 5 reviews rated 5 using 933-ZN in the following applications:
Scientific Data Images for Recombinant Human ACE-2 Protein, CF
Recombinant Human ACE‑2 Protein SEC-MALS.
Recombinant Human ACE-2/His (Catalog # 933-ZN) has a molecular weight (MW) of 189.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Multiple Lots of Recombinant Human ACE-2 bind SARS-CoV-2 Spike RBD Protein
Four independent lots of recombinant Human ACE-2 His-tag 933-ZN were tested in a functional ELISA for binding to recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein 10499-CV. All four lots were plotted on the same graph to show lot-to-lot consistency of recombinant Human ACE-2. The ED50 for this effect is 1.00-12.0 ng/mL.
SPR Data of Recombinant Human ACE-2 binding to SARS-CoV-2 Spike RBD protein
Recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein (10499-CV) was immobilized on a Biacore Sensor Chip CM5, and binding to recombinant human ACE-2 (933-ZN) was measured at a concentration range between 0.73 nM and 46.7 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of Kd=7.037 nM. (Biacore T200).
Multiple Lots of Recombinant Human ACE-2 bind SARS-CoV-2 Spike RBD Protein
Recombinant Human ACE-2 His-tag (Catalog # 933-ZN) is measured by its ability to cleave fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (ES007).
Formulation, Preparation, and Storage
933-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, ZnCl2 and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: ACE-2
References
- Kuba, K. et al. (2010) Pharmacol. Ther. 128:119.
- Yan, et al. (2020) Science 367:1444.
- Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
- Kuba, K. et al. (2005) Nature Med. 11:875.
- Hoffmann, M. et al. (2020) Cell.181:1.
- Wrapp, et al. (2020) Science 367:1260.
- Imai, Y. et al. (2005) Nature 436:112.
- Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
- Schrom, E. et al. (2017) Mol. Therapy Nuc. Acid 7:350.
- Jia, H. et al. (2016) Shock. 46:239.
- Cole-Jeffrey, C.T. et al. (2015) J. Cadiovasc. Pharmacol. 66:540.
- Towler, P. et al. (2004) J. Biol. Chem. 279:17996.
- Crackower, M.A. et al. (2002) Nature 417:822.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional ACE-2 Products
Product Documents for Recombinant Human ACE-2 Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human ACE-2 Protein, CF
For research use only
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Citations for Recombinant Human ACE-2 Protein, CF
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Application: Enzymatic activity in vitroVerified Customer | Posted 08/24/2021
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Application: Enzymatic activity in vitroVerified Customer | Posted 10/23/2020
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