Recombinant Human ADAM9 Protein, CF Summary
Product Specifications
Ala206-Asp697, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
939-AD
| Formulation | Supplied as a 0.2 μm filtered solution in MES and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Assay Procedure
- Assay Buffer: 25 mM Tris, 2.5 µM ZnCl2, 0.005% (w/v) Brij-35, pH 9.0
- Recombinant Human ADAM-9 (rhADAM9) (Catalog # 939-AD)
- Fluorogenic Peptide Substrate III: MCA-Pro-Leu-Ala-Gln-Ala-Val-DPA-Arg-Ser-Ser-Ser-Arg-NH2 (Catalog # ES003)
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rhADAM9 to 40 µg/mL in Assay Buffer.
- Dilute substrate to 20 µM in Assay Buffer.
- Combine equal volumes of 40 µg/mL rhADAM9 and 20 µM substrate. Include a control containing Assay Buffer and 20 µM substrate without any rhADAM9.
- Incubate reactions at 37 °C for 30 minutes.
- Load 100 μL of reactions and control per well in a black plate.
- Read with excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively, in endpoint mode.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Fluorescence* (RFU) x Conversion Factor** (pmol/RFU) |
| Incubation time (min) x amount of enzyme (µg) |
*Adjusted for Control
**Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).
- rhADAM9: 2.0 µg
- Substrate: 10 µM
Reconstitution Calculator
Background: ADAM9
ADAM9, also known as MDC9 or meltrin gamma, is a member of the ADAM family that contains a disintegrin and metalloprotease‑like domain (1). Like other membrane-anchored ADAMs, ADAM9 consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc‑binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. ADAM9 is able to cleave peptides corresponding to cleavage sites of tumor necrosis factor-alpha (TNF-alpha ), the p75 TNF receptor, the beta -amyloid protein precursor, and the c‑kit ligand-1, implying that it may participate in shedding of these membrane proteins (2). In fact, ADAM9 has been shown to shed membrane‑anchored heparin-binding EGF-like growth factor (3). In addition, it also cleaves oxidized insulin beta -chain and fibronectin (2,4). Besides its catalytic activity, ADAM9 functions as an adhesion molecule through binding of its disintegrin domain to integrins such as alpha v beta 5 and alpha 6 beta 1 (5, 6). The cytoplasmic domain of ADAM9 interacts with Src homology 3
(SH3)‑containing proteins and protein kinase C, and may mediate different signaling pathways (3, 7). ADAM9 is widely expressed in tissues (8).
- Moss, et al. (2001) DDT 6:417.
- Roghan, et al. (1999) J. Biol. Chem. 274:3531.
- Izumi, et al. (1998) EMBO J. 17:7260.
- Schwettmann and Tschesche (2001) Prot. Expre. & Purif. 21:65.
- Nath, et al. (2000) J. Cell Sci. 113:2319.
- Zhou, et al. (2001) Biochem. Biophys. Res. Comm. 280:574.
- Howard, et al. (1999) J. Biol. Chem. 274:31693.
- Weskamp, et al. (1996) J. Cell. Biol. 132:717.
Citations for Recombinant Human ADAM9 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
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A Novel Class of Human ADAM8 Inhibitory Antibodies for Treatment of Triple-Negative Breast Cancer
Authors: Mineva, ND;Pianetti, S;Das, SG;Srinivasan, S;Billiald, NM;Sonenshein, GE;
Pharmaceutics
Species: Human
Sample Types: Serum, Cell Culture Supernates
Applications: ELISA Capture -
Preclinical Evaluation of IMGC936, a Next Generation Maytansinoid-based Antibody-drug Conjugate Targeting ADAM9-expressing Tumors
Authors: JA Scribner, SW Hicks, KW Sinkeviciu, NC Yoder, G Diedrich, JG Brown, J Lucas, ME Fuller, T Son, A Dastur, J Hooley, CW Espelin, M Themeles, FZ Chen, Y Li, M Chiechi, J Lee, B Barat, L Widjaja, S Gorlatov, J Tamura, V Ciccarone, O Ab, KA McEachem, S Koenig, EH Westin, PA Moore, T Chittenden, RJ Gregory, E Bonvini, D Loo
Molecular Cancer Therapeutics, 2022-07-05;0(0):.
Species: Human
Sample Types: Recombinant Proteins
Applications: ELISA Capture -
The ADAM9/UBN2/AKR1C3 axis promotes resistance to androgen-deprivation in prostate cancer
Authors: TT Le, CL Hsieh, IH Lin, CY Chu, AD Do, SH Chen, K Shigemura, K Kitagawa, M Fujisawa, MC Liu, KC Chen, SY Sung
American journal of cancer research, 2022-01-15;12(1):176-197.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Novel therapeutic features of disulfiram against hepatocellular carcinoma cells with inhibitory effects on a disintegrin and metalloproteinase 10
Authors: K Goto, J Arai, A Stephanou, N Kato
Oncotarget, 2018-04-10;9(27):18821-18831.
Applications: Enzyme Assay -
Deletion of ADAM-9 in HGF/CDK4 mice impairs melanoma development and metastasis
Authors: N Giebeler, A Schönefu beta, J Landsberg, T Tüting, C Mauch, P Zigrino
Oncogene, 2017-05-29;0(0):.
Applications: Enzyme Assay -
ADAM8 as a drug target in pancreatic cancer.
Authors: Schlomann U, Koller G, Conrad C, Ferdous T, Golfi P, Garcia A, Hofling S, Parsons M, Costa P, Soper R, Bossard M, Hagemann T, Roshani R, Sewald N, Ketchem R, Moss M, Rasmussen F, Miller M, Lauffenburger D, Tuveson D, Nimsky C, Bartsch J
Nat Commun, 2015-01-28;6(0):6175.
Species: Primate - Chlorocebus aethiops (African Green Monkey)
Sample Types: Whole Cells
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ADAM9 is a novel product of polymorphonuclear neutrophils: regulation of expression and contributions to extracellular matrix protein degradation during acute lung injury.
Authors: Roychaudhuri R, Hergrueter A, Polverino F, Laucho-Contreras M, Gupta K, Borregaard N, Owen C
J Immunol, 2014-07-25;193(5):2469-82.
Species: Human
Sample Types: Protein, Whole Cells
Applications: Bioassay, Enzyme Assay -
The ADAM10 prodomain is a specific inhibitor of ADAM10 proteolytic activity and inhibits cellular shedding events.
Authors: Moss ML, Bomar M, Liu Q, Sage H, Dempsey P, Lenhart PM, Gillispie PA, Stoeck A, Wildeboer D, Bartsch JW, Palmisano R, Zhou P
J. Biol. Chem., 2007-09-25;282(49):35712-21.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Targeting ADAM-mediated ligand cleavage to inhibit HER3 and EGFR pathways in non-small cell lung cancer.
Authors: Zhou BB, Peyton M, He B, Liu C, Girard L, Caudler E, Lo Y, Baribaud F, Mikami I, Reguart N, Yang G, Li Y, Yao W, Vaddi K, Gazdar AF, Friedman SM, Jablons DM, Newton RC, Fridman JS, Minna JD, Scherle PA
Cancer Cell, 2006-07-01;10(1):39-50.
Species: Human
Sample Types:
Applications: Enzyme Assay -
ADAM-9 (MDC-9/meltrin-gamma), a member of the a disintegrin and metalloproteinase family, regulates myeloma-cell-induced interleukin-6 production in osteoblasts by direct interaction with the alpha(v)beta5 integrin.
Authors: Karadag A, Zhou M, Croucher PI
Blood, 2005-12-22;107(8):3271-8.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Selective roles for tumor necrosis factor alpha-converting enzyme/ADAM17 in the shedding of the epidermal growth factor receptor ligand family: the juxtamembrane stalk determines cleavage efficiency.
Authors: Hinkle CL, Sunnarborg SW, Loiselle D, Parker CE, Stevenson M, Russell WE, Lee DC
J. Biol. Chem., 2004-04-05;279(23):24179-88.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Enzyme Assay
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