Recombinant Human ADAMTS-L2 His-tag Protein, CF

Catalog #: 10950-AD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10950-AD-020

Recombinant Human ADAMTS-L2 His-tag Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human ADAMTS-L2 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human ADAMTSL2 His-tag (Catalog# 10950-AD) immobilized at 2.00 μg/mL, 100 μL/well, the concentration of Recombinant Human Lysyl Oxidase Homolog 2 Protein (Catalog # 2639-AO) that produces 50% of the optimal binding response is approximately 0.60-3.00 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human ADAMTSL2 protein
Asp23-Ser951 with a C-terminal 6-His tag

Accession #

Q86TH1.1

N-terminal Sequence
Analysis

Asp23

Predicted Molecular Mass

103 kDa

SDS-PAGE

130-144 kDa, under reducing conditions

Product Datasheets

10950-AD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10950-AD

Formulation	Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human ADAMTSL2 His-tag (Catalog# 10950-AD) is immobilized at 2.00 μg/mL, 100 μL/well, the concentration of Recombinant Human Lysyl Oxidase Homolog 2 Protein (2639-AO) that produces 50% of the optimal binding response is approximately 0.60-3.00 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human ADAMTS-L2 His-tag Protein (Catalog # 10950-AD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 130-144 kDa.

Reconstitution Calculator

Background: ADAMTSL2

ADAMTSL2 (a disintegrin and metalloproteinase with thrombospondin motifs like 2), is one protein from a seven-member family of ADAMTS-like secreted proteins that are related to ADAMTS zinc metalloproteases but do not contain a metalloprotease domain. ADAMTSL2 contains a multidomain structure that includes an N-terminal signal sequence, C-terminal thrombospondin repeats, ADAMTS cysteine-rich and spacer modules, a PLAC domain and an N-glycan rich module unique amongst ADAMTSL family members (1). These domains play a substantial role in ADAMTS specificity and ADAMTSLs are also expected to have specific extracellular ligands. ADAMTSL2 is abundantly expressed in liver, lung, and spleen and resides in the extracellular matrix (ECM) (2). It has been shown to interact with many ECM proteins including fibrillin microfibrils (3), members of the lysyl oxidase family such as LOXL2 (4), and latent transforming growth factor beta binding protein 1 (LTBP1) (5). The interaction between ADAMTSL2 and LTBP1 and fibrillin indicates ADAMTSL2 may have a role in modulation of microfibrillar formation and function (3,5) and ADAMTSL2 has been found upregulated in cardiac fibrosis (6). Through its interaction with the LTBP1 protein and lysyl oxidase family members, ADAMTSL2 protein has been suggested to regulate the availability of TGF-beta and consequent TGF-beta signaling (1,5). Several mutations of ADAMTSL2 lead to a heterogeneous autosomal recessive disease, Geleophysic dysplasia 1 (7, 8). Geleophysic dysplasia 1 may be specifically caused by impaired secretion of ADAMTSL2 due to glycosylation differences (9). A variant of ADAMTSL2 has also been shown to result in an autosomal dominant inherited connective tissue disorder consistent with Ehlers-Danlos syndrome (10).

References