Recombinant Human ALCAM/CD166 His-tag Avi-tag Protein, CF

Catalog #: AVI11191 Datasheet / COA / SDS

Biotinylated

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AVI11191-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human ALCAM/CD166 His-tag Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD6 Fc Chimera (Catalog # 627-CD) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human ALCAM/CD166 His-tag Avi-tag (Catalog # AVI11191) binds with an ED₅₀ of 20.0-200 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human ALCAM/CD166 protein

Human ALCAM/CD166 (Trp28-Ala526) Accession # Q13740.2	6-His tag	Avi-tag
N-terminus		C-terminus

Accession #

Q13740.2

N-terminal Sequence
Analysis

Trp28

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

59 kDa

SDS-PAGE

71-90 kDa, under reducing conditions.

Product Datasheets

AVI11191

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11191

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human CD6 Fc Chimera (627-CD) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human ALCAM/CD166 His-tag Avi-tag Protein (Catalog # AVI11191) binds with an ED₅₀ of 20.0-200 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human ALCAM/CD166 His-tag Avi-tag Protein (Catalog # AVI11191) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 71-90 kDa.

Reconstitution Calculator

Background: ALCAM/CD166

Activated leukocyte cell adhesion molecule (ALCAM), also known as CD166, is a surface glycoprotein belonging to the immunoglobulin superfamily (1, 2). ALCAM, along with MCAM and BCAM/Lu, form a small subgroup of adhesion molecules involved in tissue development and maintenance, neurogenesis, and regulation of immune responses (3). Mature human ALCAM consists of an extracellular domain (ECD) containing 2 IgV and 3 IgC domains, a transmembrane domain, and a short cytoplasmic domain (1, 2). Within the ECD, human ALCAM shares 93% amino acid sequence identity with both mouse and rat ALCAM. An isoform of ALCAM with a shorter stalk region in the ECD is known to exist and is associated with higher shedding tendency and decreased cell adhesion (2). Initially found expressed on activated leukocytes, ALCAM expression has been detected in a wide variety of tissues and cells (3, 4). In addition to cell adhesion, other ALCAM functions include leukocyte migration across the blood brain barrier, T-cell activation, and osteogenesis, (2, 5, 6). Overexpression of ALCAM is often associated with poor prognosis in various human tumors, such as bladder cancer, prostate cancer, melanoma, and liver cancer (2, 7). Our Avi-tag Biotinylated Human ALCAM/CD166 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References