Recombinant Human alpha 2-Macroglobulin-like 1 Protein, CF
Recombinant Human alpha 2-Macroglobulin-like 1 Protein, CF Summary
Product Specifications
Glu18-Glu1454, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
7836-AM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: alpha 2-Macroglobulin-like 1/A2ML1
alpha 2-Macroglobulin like-1 (A2ML1) is an approximately 180 kDa secreted glycoprotein that is structurally and functionally similar to alpha 2‑Macroglobulin (A2M) (1). Mature human A2ML1 shares 40% amino acid sequence identity with human A2M. It functions as a broad spectrum protease inhibitor with activity toward chymotrypsin, papain, thermolysin, and subtilisin A (2). Like A2M, A2ML1 binds to proteases covalently and contains a bait region (2). When the bait region is cleaved by a targeted protease, A2M undergoes a conformational change which blocks the protease’s active site and also promotes A2M clearance from the circulation (1). In contrast to the dimeric and tetrameric A2M, A2ML1 is secreted as a monomer (2, 3). A2ML1 is expressed in epidermis, testis, thymus, and placenta (2). It binds to Kallikrein 7, a protease that regulates epidermal desquamation by disrupting corneocyte adhesion (2, 4). The C‑terminal domain of A2ML1 mediates binding to LRP1 on the surface of macrophages followed by endocytosis (5). A2M has a similar receptor‑binding domain which is responsible for A2M interactions with LRP1 (6). A2ML1 is also known as the keratinocyte p170 autoantigen in the skin disease paraneoplastic pemphigus (7). During zebrafish development, an A2ML1-like protein is required for liver formation (8).
- Sottrup-Jensen, L. (1989) J. Biol. Chem. 264:11539.
- Galliano, M.-F. et al. (2006) J. Biol. Chem. 281:5780.
- Gettins, P.G. et al. (1995) J. Biol. Chem. 270:14160.
- Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.
- Galliano, M.-F. et al. (2008) PLoS ONE 3:e2729.
- Sottrup-Jensen, L. et al. (1986) FEBS Lett. 205:20.
- Schepens, I. et al. (2010) PLoS ONE 5:e12250.
- Hong, S.-K. and I.B. Dawid (2008) PLoS ONE 3:e3736.
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human alpha 2-Macroglobulin-like 1 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human alpha 2-Macroglobulin-like 1 Protein, CF and earn rewards!
Have you used Recombinant Human alpha 2-Macroglobulin-like 1 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
