Recombinant Human AMIGO2 Fc Chimera Protein, CF Summary
Product Specifications
| Human AMIGO2 (Gly39-His393) Accession # Q86SJ2 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2080-AM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: AMIGO2
AMIGO2 (amphoterin‑induced gene and ORF 2), also known as DEGA and Alivin‑1, is an approximately 65 kDa transmembrane cell adhesion protein. It belongs to a family of leucine‑rich repeat (LRR) containing proteins that play various roles in nervous system development and function (1, 2). Mature human AMIGO2 consists of a 359 amino acid (aa) extracellular domain (ECD) with six LRRs flanked by single LRRNT and LRRCT domains, followed by one immunoglobulin-like domain, a 21 aa transmembrane segment, and a 103 aa cytoplasmic domain (3‑5). Within the ECD, human AMIGO2 shares 89% and 87% aa sequence identity with mouse and rat AMIGO2, respectively. AMIGO2 forms homodimers as well as heterodimers with the related AMIGO and AMIGO3 molecules (3, 6). Within the nervous system, AMIGO2 is transcribed in the cerebrum, hypothalamus, olfactory bulb, retina, hippocampus (pyramidal cells), and cerebellum (granule neurons and Purkinje cells) (3, 4, 7). It is also expressed in the liver, lung, testis, spleen, and small intestine (3, 4). AMIGO2 supports neuron survival, and is down‑regulated following pro‑apoptotic stimulation (4). Its expression can be up‑regulated or down‑regulated in a variety of cancers, and anti‑sense knockdown of AMIGO2 interferes with tumor cell adhesion to collagen as well as in vivo tumorigenicity (5).
- de Wit, J. et al. (2011) Annu. Rev. Cell Dev. Biol. 27:697.
- Chen, Y. et al. (2006) Brain Res. Rev. 51:265.
- Kuja-Panula, J. et al. (2003) J. Cell Biol. 160:963.
- Ono, T. et al. (2003) J. Neurosci. 23:5887.
- Rabenau, K.E. et al. (2004) Oncogene 23:5056.
- Kajander, T. et al. (2011) J. Mol. Biol. 413:1001.
- Laeremans, A. et al. (2013) Brain Struct. Funct. 218:123.
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