Recombinant Human Angiopoietin-like 5 Protein, CF
Recombinant Human Angiopoietin-like 5 Protein, CF Summary
Product Specifications
Met1-Lys388 with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6675-AN/CF
| Formulation | Supplied as a 0.2 μm filtered solution in PBS and NaCl. |
| Reconstitution | |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human Angiopoietin-like Protein 5/ANGPTL5 (Catalog # 6675-AN/CF) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (2078-T4) binds with an ED50 of 80.0-800 ng/mL.
Reconstitution Calculator
Background: Angiopoietin-like Protein 5/ANGPTL5
Angiopoietin-like 5 (ANGPTL5) is a secreted glycoprotein that is structurally related to the angiopoietins which contain an N-terminal coiled‑coil domain and a C-terminal fibrinogen-like domain (1-3). It shares 88% and 93% aa sequence identity with bovine and porcine ANGPTL5, and greater than 98% identity with chimpanzee, orangutan, and rhesus ANGPTL5. ANGPTL5 is expressed in adipose, bronchial, epididymal, and heart tissue (3, 4). Rare polymorphisms and loss of function mutations in human ANGPTL5 are associated with low circulating triglyceride levels and alterations in body mass index (4, 5). ANGPTL5, when used in combination with other growth factors such as SCF, Thrombopoietin, IGF-II, FGF acidic, Flt-3 Ligand, and IGFBP-2, enhances the expansion and engraftment of human and mouse hematopoietic stem cells (6-8). The coiled-coil domain of ANGPTL proteins is critical for this effect. However, the molecular mechanism of ANGPTL proteins in stem cell activity remains unclear (6).
- Oike, Y. et al. (2009) Circ. J. 73:2192.
- Miida, T. and S. Hirayama (2010) Curr. Opin. Lipidol. 21:70.
- Zeng, L. et al. (2003) J. Hum. Genet. 48:159.
- Romeo, S. et al. (2009) J. Clin. Invest. 119:70.
- Liu, D.J. and S.M. Leal (2010) PloS Genet. 6:e1001156.
- Zhang, C.C. et al. (2006) Nat. Med. 12:240.
- Zhang, C.C. et al. (2008) Blood 111:3415.
- Khoury, M. et al. (2010) Stem Cells Dev. Dec 8 epub.
FAQs
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This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?
The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.
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