Home / Angiopoietin-like Protein 5/ANGPTL5 / Recombinant Human Angiopoietin-like 5 Protein, CF

Recombinant Human Angiopoietin-like 5 Protein, CF

Catalog #: 6675-AN/CF Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
6675-AN-025/CF
Recombinant Human Angiopoietin‑like Protein 5/ANGPTL5 Binding Activity.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Angiopoietin-like 5 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Angiopoietin-like Protein 5/ANGPTL5 (Catalog # 6675-AN/CF) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078-T4) binds with an ED50 of 80.0-800 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 5/ANGPTL5 protein
Met1-Lys388 with a C-terminal 6-His tag
Accession #
AAH49170
N-terminal Sequence
Analysis
Val23 & Asn26
Predicted Molecular Mass
42.3 kDa
SDS-PAGE
50-60 kDa, reducing conditions

Product Datasheets

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6675-AN/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6675-AN/CF

Formulation Supplied as a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Human Angiopoietin-like Protein 5/ANGPTL5 (Catalog # 6675-AN/CF) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (2078-T4) binds with an ED50 of 80.0-800 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 5/ANGPTL5

Angiopoietin-like 5 (ANGPTL5) is a secreted glycoprotein that is structurally related to the angiopoietins which contain an N-terminal coiled‑coil domain and a C-terminal fibrinogen-like domain (1-3). It shares 88% and 93% aa sequence identity with bovine and porcine ANGPTL5, and greater than 98% identity with chimpanzee, orangutan, and rhesus ANGPTL5. ANGPTL5 is expressed in adipose, bronchial, epididymal, and heart tissue (3, 4). Rare polymorphisms and loss of function mutations in human ANGPTL5 are associated with low circulating triglyceride levels and alterations in body mass index (4, 5). ANGPTL5, when used in combination with other growth factors such as SCF, Thrombopoietin, IGF-II, FGF acidic, Flt-3 Ligand, and IGFBP-2, enhances the expansion and engraftment of human and mouse hematopoietic stem cells (6-8). The coiled-coil domain of ANGPTL proteins is critical for this effect. However, the molecular mechanism of ANGPTL proteins in stem cell activity remains unclear (6).

References
  1. Oike, Y. et al. (2009) Circ. J. 73:2192.
  2. Miida, T. and S. Hirayama (2010) Curr. Opin. Lipidol. 21:70.
  3. Zeng, L. et al. (2003) J. Hum. Genet. 48:159.
  4. Romeo, S. et al. (2009) J. Clin. Invest. 119:70.
  5. Liu, D.J. and S.M. Leal (2010) PloS Genet. 6:e1001156.
  6. Zhang, C.C. et al. (2006) Nat. Med. 12:240.
  7. Zhang, C.C. et al. (2008) Blood 111:3415.
  8. Khoury, M. et al. (2010) Stem Cells Dev. Dec 8 epub.
Entrez Gene IDs
253935 (Human)
Alternate Names
Angiopoietin like Protein 5; angiopoietin-like 5; Angiopoietin-like Protein 5; angiopoietin-related protein 5; ANGPTL5

FAQs

  1. This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    • The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

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