Recombinant Human APLP-1 Protein, CF

Catalog #: 9605-AL Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9605-AL-050

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Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Human APLP-1 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Mouse myeloma cell line, NS0-derived human APLP-1 protein
Gln34-Glu580, with a C-terminal 6-His tag

Accession #

P51693-1

N-terminal Sequence
Analysis

Gln34 (blocked)

Predicted Molecular Mass

62 kDa

SDS-PAGE

74-89 kDa, reducing conditions

Product Datasheets

9605-AL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9605-AL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: APLP-1

Amyloid Precursor-like Proteins (APLPs) are members of the amyloid precursor protein (APP) superfamily of transmembrane proteins. APLP-1, APLP-2, and APP share highly homologous amino acid sequences, except that APLPs lack the beta -amyloid domain derived by proteolytic processing from APP (1). APLP-1 is expressed post-synaptically in cortical neurons (2). APLP-1 is post-translationally modified by N-glycosylation and displays an apparent molecular mass of 80-90 kDa in reducing SDS-PAGE (3). Similar to APP and APLP-2, APLP-1 is susceptible to cleavage by MMPs and secretases, generating multiple fragments from the extracellular and intracellular domains (4). These include peptides similar to the amyloidogenic A beta peptides and a cytoplasmic fragment that associates with Fe65 family proteins and functions as a transcriptional activator (4). The extracellular domain contains heparin and collagen binding regions. human APLP-1 shares 88% and 86% aa sequence identity with mouse and rat APLP-1, respectively.

References