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Recombinant Human APPBP1/UBA3 Complex (NEDD8 E1) Protein, CF

Catalog #: E-313
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
E-313-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (8)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human APPBP1/UBA3 Complex (NEDD8 E1) Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human APPBP1/UBA3 Complex (NEDD8 E1) Protein is a member of the NEDD8-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a NEDD8-conjugating (E2) enzyme and a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human APPBP1/UBA3 Complex (NEDD8 E1) Protein concentration of 50-200 nM.
Source
E. coli-derived human APPBP1/UBA3 Complex protein
Met1 - Leu534 (APPBP1) Met22 - Ser463 (UBA3)
Accession #
Q13564.1 (APPBP1) Q8TBC4.2 (UBA3)
Predicted Molecular Mass

60 kDa (APPBP1)

50 kDa (UBA3)

Product Datasheets

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E-313

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-313

Formulation Supplied as a solution in HEPES and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: APPBP1/UBA3 Complex

Recombinant Human APPBP1/UBA3 Complex (NEDD8 Activating Enzyme) is a heterodimeric enzyme with a predicted molecular weight of 112 kDa. It is responsible for the first step in the conjugation of NEDD8 to protein substrates. The heterodimer is composed of a regulatory subunit, Amyloid beta Precursor Protein Binding Protein 1 (APP-BP1), and a catalytic subunit, Ubiquitin-like Modifier Activating Enzyme 3 (UBA3). Human APP-BP1 is a 534 amino acid (aa) protein with a predicted molecular weight of 60 kDa that is expressed ubiquitously in fetal tissues and in the adult brain (1). APP-BP1 is required for UBA3 neddylation activity, regulates enzyme specificity, and is expressed as two isoforms, the full length protein and a second isoform with an alternate N-terminal, aa1-17, sequence (2). APP-BP1 has been shown to drive cell cycle progression, and its expression is increased in the hippocampus of Alzheimer's disease brains (3,4). Human UBA3 is a 463 aa protein with a predicted molecular weight of 52 kDa. It is ubiquitously expressed and shares high aa sequence identity with the C-terminal domain of human UBE1 (5). UBA3 contains an ATP-binding domain and an active site cysteine residue, Cys237 in humans, which are both common to E1 enzymes. Like APP-BP1, two isoforms of UBA3 have been identified in humans, the full length protein and a truncated isoform, which lacks aa 8-21. UBA3 is required for cell cycle progression and has been shown to downregulate steroid receptor activation (4,6). Neddylation and its associated enzymes have been implicated in the progression of Alzheimer's disease, via neddylation of APP, and cancer via post-translational modification of oncogenes (7,8).

References
  1. Chow, N. et al. (1996) J. Biol. Chem. 271:11339.
  2. Walden, H. et al. (2003) Mol. Cell 12:1427.
  3. Chen, Y. et al. (2003) J. Cell Biol. 163:27.
  4. Chen, Y. et al. (2000) J. Biol. Chem. 275:8929.
  5. Gong, L. & E.T. Yeh (1999) J. Biol. Chem. 274:12036.
  6. Fan, M. et al. (2002) Mol. Endocrinol. 16:315.
  7. Chen, Y. et al. (2012) J. Cell Mol. Med. 16:2583.
  8. Soucy, T.A. et al. (2010) Genes Cancer 1:708.
Long Name
Amyloid beta Precursor Protein-binding Protein 1/Ubiquitin Like Modifier Activating Enzyme 3
Entrez Gene IDs
8883 (Human); 234664 (Mouse); 84019 (Rat)
Alternate Names
A-116A10.1; amyloid beta precursor protein binding protein 1; amyloid beta precursor protein binding protein 1, 59kDa; Amyloid beta precursor protein-binding protein 1, 59 kDa; amyloid beta precursor protein-binding protein 1, 59kD; Amyloid protein-binding protein 1; APPBP1/UBA3 Complex; APPBP1APP-BP1; NEDD8 activating enzyme E1 subunit 1; NEDD8-activating enzyme E1 regulatory subunit; NEDD8-activating enzyme E1 subunit; protooncogene protein 1; Proto-oncogene protein 1; ula-1

Citations for Recombinant Human APPBP1/UBA3 Complex (NEDD8 E1) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Mono-ubiquitination empowers ubiquitin chain elongation
    Authors: Wu, K;DeVita, RJ;Pan, ZQ;
    The Journal of biological chemistry
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Targeting a helix-in-groove interaction between E1 and E2 blocks ubiquitin transfer
    Authors: AM Cathcart, GH Bird, TE Wales, HD Herce, EP Harvey, ZJ Hauseman, CE Newman, U Adhikary, MS Prew, T Oo, S Lee, JR Engen, LD Walensky
    Nat. Chem. Biol., 2020-08-17;0(0):.
    Species: Human, N/A
    Sample Types: Peptide, Protein
    Applications: Bioassay
  3. Mdm2-mediated neddylation of pVHL blocks the induction of antiangiogenic factors
    Authors: ER Wolf, AR Mabry, B Damania, LD Mayo
    Oncogene, 2020-06-17;0(0):.
    Species: Human
    Sample Types: In Vitro
    Applications: Bioassay
  4. Discovery of Ubiquitin Deamidases in the Pathogenic Arsenal of Legionella pneumophila
    Authors: D Valleau, AT Quaile, H Cui, X Xu, E Evdokimova, C Chang, ME Cuff, ML Urbanus, S Houliston, CH Arrowsmith, AW Ensminger, A Savchenko
    Cell Rep, 2018-04-10;23(2):568-583.
    Applications: Bioassay
  5. A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation
    Authors: H Zhou, J Lu, L Liu, D Bernard, CY Yang, E Fernandez-, K Chinnaswam, S Layton, J Stuckey, Q Yu, W Zhou, Z Pan, Y Sun, S Wang
    Nat Commun, 2017-10-27;8(1):1150.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  6. SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function
    Authors: KE Coleman, M Békés, JR Chapman, SB Crist, MJ Jones, BM Ueberheide, TT Huang
    Elife, 2017-05-05;6(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. The ubiquitin-associated (UBA) domain of SCCRO/DCUN1D1 protein serves as a feedback regulator of biochemical and oncogenic activity.
    Authors: Huang G, Towe C, Choi L, Yonekawa Y, Bommelje C, Bains S, Rechler W, Hao B, Ramanathan Y, Singh B
    J Biol Chem, 2014-11-19;290(1):296-309.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  8. SCCRO3 (DCUN1D3) antagonizes the neddylation and oncogenic activity of SCCRO (DCUN1D1).
    Authors: Huang G, Stock C, Bommelje C, Weeda V, Shah K, Bains S, Buss E, Shaha M, Rechler W, Ramanathan S, Singh B
    2014-10-27;0(0):.
    Species: Human
    Sample Types: Cell Lysates

FAQs

  1. Is there a linker sequence between the APPBP1 and UBA3 subunits?  

    •  The two subunits of this protein (APPBP1 and UBA3) are not linked.

View all Proteins and Enzyme FAQs

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