Recombinant Human B7-H3 Fc Chimera Protein, CF Summary
Product Specifications
Optimal dilutions should be determined by each laboratory for each application.
| Human B7-H3 (Leu29-Pro245) Accession # NP_079516 |
DIEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1027-B3
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: B7-H3
Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for regulating immune responses (1‑3). Other family members include B7-1, B7-2, B7-H2, PD-L1 (B7-H1), and PD-L2. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share about 20-40% amino acid (aa) sequence identity. The cloned human B7-H3 cDNA encodes a 316 aa type I membrane precursor protein with a putative 28 aa signal peptide, a 217 aa extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a 45 aa cytoplasmic domain. An isoform of human B7-H3 containing a four-Ig-like domain extracellular region has also been identified. Human B7-H3 is not expressed on resting B cells, T cells, monocytes or dendritic cells, but is induced on dendritic cells and monocytes by inflammatory cytokines. B7-H3 expression is also detected on various normal tissues and in some tumor cell lines. Human B7-H3 does not bind any known members of the CD28 family of immunoreceptors. However, B7-H3 has been shown to bind an unidentified counter-receptor on activated T cells to costimulate the proliferation of CD4+ or CD8+ T cells. B7-H3 has also been found to enhance the induction of primary cytotoxic T lymphocytes and stimulate IFN-gamma production (1-3).
- Chapoval, A.I. et al. (2001) Nat. Immunol. 2:269.
- Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
- Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
Citations for Recombinant Human B7-H3 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Clinical significance of B7-H3 expression in circulating CD4+CD25high T cells, CD14+ monocytes, and plasma for the progression of HIV infection
Authors: Xu, JC;Chen, H;Xu, P;You, XR;Zhu, GC;Gao, F;
BMC infectious diseases
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Targeting brain lesions of non-small cell lung cancer by enhancing CCL2-mediated CAR-T cell migration
Authors: H Li, EB Harrison, H Li, K Hirabayash, J Chen, QX Li, J Gunn, J Weiss, B Savoldo, JS Parker, CV Pecot, G Dotti, H Du
Nature Communications, 2022-04-20;13(1):2154.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Delivery of CAR-T cells in a transient injectable stimulatory hydrogel niche improves treatment of solid tumors
Authors: AK Grosskopf, L Labanieh, DD Klysz, GA Roth, P Xu, O Adebowale, EC Gale, CK Jons, JH Klich, J Yan, CL Maikawa, S Correa, BS Ou, AI d'Aquino, JR Cochran, O Chaudhuri, CL Mackall, EA Appel
Science Advances, 2022-04-08;8(14):eabn8264.
Species: Human
Sample Types: Whole Cell
Applications: Flow Cytometry -
Proinflammatory cytokines promote TET2-mediated DNA demethylation during CD8 T�cell effector differentiation
Authors: CC Zebley, HA Abdelsamed, HE Ghoneim, S Alli, C Brown, D Haydar, T Mi, T Harris, MA McGargill, G Krenciute, B Youngblood
Cell Reports, 2021-10-12;37(2):109796.
Species: Human
Sample Types: Transduced Whole Cells
Applications: Bioassay -
Targeting Radiation-Resistant Prostate Cancer Stem Cells by B7-H3 CAR T Cells
Authors: Y Zhang, L He, A Sadagopan, T Ma, G Dotti, Y Wang, H Zheng, X Gao, D Wang, AB DeLeo, S Fan, R Sun, L Yu, L Zhang, G Wang, S Ferrone, X Wang
Molecular Cancer Therapeutics, 2021-03-01;20(3):577-588.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Evaluation of Prognostic Immune Signatures in Patients with Breast, Colorectal and Pancreatic Cancer Receiving Chemotherapy
Authors: UM Vogl, L Öhler, M Rasic, JM Frischer, M Modak, J Stöckl
Anticancer Res., 2017-04-01;37(4):1947-1955.
Applications: ELISA (Standard) -
Preferential Induction of the T Cell Auxiliary Signaling Molecule B7-H3 on Synovial Monocytes in Rheumatoid Arthritis
Authors: BR Yoon, YH Chung, SJ Yoo, K Kawara, J Kim, IS Yoo, CG Park, SW Kang, WW Lee
J. Biol. Chem, 2015-12-23;291(8):4048-57.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
B7-h3 augments the inflammatory response and is associated with human sepsis.
Authors: Zhang G, Wang J, Kelly J
J. Immunol., 2010-08-09;185(6):3677-84.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Tumor cell and tumor vasculature expression of B7-H3 predict survival in clear cell renal cell carcinoma.
Authors: Crispen PL, Sheinin Y, Roth TJ, Lohse CM, Kuntz SM, Frigola X, Thompson RH, Boorjian SA, Dong H, Leibovich BC, Blute ML, Kwon ED
Clin. Cancer Res., 2008;14(16):5150-7.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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Average Rating: 5 (Based on 1 Review)
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Reason for Rating: Worked perfectly in ELISA analysis of antibody production of cell fusions.
