Recombinant Human BAI3 Protein, CF

Catalog #: 9106-BA Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9106-BA-050

Recombinant Human BAI3 Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human BAI3 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL3 (Catalog # 9115-TN) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI3 that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human BAI3 protein
Ala25-Thr880, with C-terminal 6-His tag

Accession #

NP_001695

N-terminal Sequence
Analysis

Ala25

Predicted Molecular Mass

97 kDa

SDS-PAGE

109-132 kDa, reducing conditions

Product Datasheets

9106-BA

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9106-BA

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Human C1qL3 (Catlog # 9115-TN) is coated at 1 µg/mL (100 μL/well), Recombinant Human BAI3 (Catlog # 9106-BA) binds with a typical ED₅₀ of 0.3-1.8 μg/mL.

Reconstitution Calculator

Background: BAI3

Brain angiogenesis inhibitor 3 (BAI3) is an approximately 180 kDa adhesion GPCR that plays a role in neuronal synapse formation and maintenance. Mature human BAI3 consists of an 856 amino acid (aa) N-terminal extracellular domain (ECD) with one CUB domain, four TSP1 domains, and a GPS protease sensitive linker, followed by a region with seven transmembrane segments and a 376 aa C-terminal cytoplasmic domain (1, 2). Within the N-terminal ECD, human BAI3 shares 98% and 97% aa sequence identity with mouse and rat BAI3, respectively. BAI3 is primarily expressed post-synaptically in the cerebral cortex and on cerebellar Purkinje cells (1-5), and it binds to the TNF-like proteins C1qL1, 2, 3, and 4 (6). BAI3 interaction with C1qL1 is required for the formation and maintenance of excitatory synapses between climbing fibers and parallel fibers with Purkinje cells (4, 5). BAI3 ligation can also reduce excitatory synaptic density in hippocampal neurons, decrease dendrite arborization, and promote axon pruning in climbing fibers (3, 4, 6). It is additionally expressed in developing muscle where it is required for myoblast fusion (7).

References