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Recombinant Human BCAM His-tag Protein, CF

Catalog #: 11173-BC Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
11173-BC-050
Recombinant Human BCAM His-tag Protein Bioactivity.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human BCAM His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of TE‑85 human osteogenic sarcoma cells. The ED50 for this effect is 0.250-3.00 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human BCAM protein
Glu32-Ala547, with a C-terminal 6-His tag
Accession #
CAA58449.1
N-terminal Sequence
Analysis
Glu32
Predicted Molecular Mass
57 kDa
SDS-PAGE
67-82 kDa, under reducing conditions.

Product Datasheets

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11173-BC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11173-BC

Formulation Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Bioactivity View Larger

Recombinant Human BCAM His-tag Protein (Catalog # 11173-BC) supports the adhesion TE‑85 human osteogenic sarcoma cells. The ED50 for this effect is 0.250-3.00 µg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human BCAM His-tag Protein (Catalog # 11173-BC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 67-82 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BCAM

Human Basal Cell Adhesion Molecule (BCAM), also known as CD239, is an immunoglobulin superfamily protein that arises from alternate splicing of the Lutheran blood group molecule (Lu). Lu and BCAM differ by a 40 amino acid (aa) SH3-containing segment that is present in the cytoplasmic domain of Lutheran (1). Mature human BCAM consists of an extracellular domain (ECD) with two Ig-like V-type domains and three Ig-like C2-type domains, a transmembrane domain, and a short cytoplasmic domain (2,3). Within the ECD, human BCAM shares 73% amino acid (aa) identity with mouse and rat BCAM. A polymorphism at position 77 within the ECD is the basis for the difference between the Lua and Lub Lutheran blood groups (4). BCAM is widely expressed in epithelial and endothelial tissues including in the vasculature, kidney glomerulus, small intestine, colon, hair follicle outer root sheath, and basal keratinocytes of the skin during inflammation (5-7). BCAM is also expressed on vascular and visceral smooth muscle cells and at the neuromuscular junction of skeletal muscle (6,8,9). Lu/BCAM binds to laminin, specifically isoforms containing the alpha 5 chain, which are found in basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation (10). Overexpression of both BCAM and Lu on sickle red blood cells (SS RBC) has been found to play a role in vaso-occlusive crisis in sickle cell patients by contributing to the adhesion of erythrocytes to the vascular wall (11,12). The adhesive role of Lu/BCAM has been studied in the context of many diseases, including sickle cell disease, hereditary spherocytosis, myeloproliferative neoplasms and Gaucher disease (13). BCAM is upregulated on carcinomas, sarcomas, astrocytomas, and melanomas (14). Additionally, Lu/BCAM has been found to assist tumor cell migration via regulation of integrin-mediated cell attachment to laminin-511 (15).

References
  1. Rahuel, C. et al. (1996) Blood 88:1865.
  2. Vainionpaa, N. et al. (2006) Am. J. Physiol. Cell Physiol. 290:C764.
  3. El Nemer, W. et al. (1997) Blood 89:4608.
  4. El Nemer, W. et al. (1999) J. Biol. Chem. 274:31903.
  5. Schon, M. et al. (2000) J. Invest. Dermatol. 115:1047.
  6. Rahuel, C. et al. (2008) Am. J. Physiol. Renal Physiol. 294:F393.
  7. Rettig, W.J. et al. (1986) Cancer Res. 46:6406.
  8. Nishimune, H. et al. (2008) J. Cell Biol. 182:1201.
  9. Kikkawa, Y. et al. (2007) J. Biol. Chem. 282:14853.
  10. El Nemer, W. et al. (2001) J Biol Chem 276:23757.
  11. Eyler, C.E. and Telen, M.J. (2006) Transfusion. 46(4).
  12. Klei, TRL. et al. (2018) Blood Adv. 2:14.
  13. Guadall, A. et al. (2019). J. Biol. Chem. 294:14911.
  14. Chang, H.Y. et al. (2017) J. Biomed Sci 24:61.
  15. Kikkawa, Y. et al. (2013) J. Biol. Chem. 288:30990.
Long Name
Basal Cell Adhesion Molecule
Entrez Gene IDs
4059 (Human); 57278 (Mouse)
Alternate Names
antigen identified by monoclonal F8; Auberger B antigen; basal cell adhesion molecule (Lu and Au blood groups); basal cell adhesion molecule (Lutheran blood group); basal cell adhesion molecule; B-CAM cell surface glycoprotein; BCAM; B-cell adhesion molecule; CD239 antigen; CD239; F8/G253 antigen; glycoprotein 95kDa; LUAU; Lutheran antigen; Lutheran blood group (Auberger b antigen included); Lutheran blood group glycoprotein; MSK19

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