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Recombinant Human BMP-10 Propeptide Protein, CF

Catalog #: 3956-BP Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
3956-BP-050
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Recombinant Human BMP-10 Propeptide Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit BMP-10-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.75-3 µg/mL in the presence of 100 ng/mL of rhBMP-10.
Source
Mouse myeloma cell line, NS0-derived human BMP-10 protein
Human BMP-10 Propeptide
(Ser20 - Arg313) & (Pro23 - Arg313)
Accession # O95393		 RR 6-His tag
N-terminus C-terminus
Accession #
O95393
N-terminal Sequence
Analysis
Ser20 & Pro23
Predicted Molecular Mass
34.6 kDa
SDS-PAGE
42-50 kDa, reducing conditions

Product Datasheets

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3956-BP

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3956-BP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-10

BMP-10, along with BMP-9, GDF-5, -6, and -7, belongs to a subgroup of sequence related TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors (1 - 3). Proteolytic removal of the propeptide from the 60 kDa proprotein yields a 12 kDa mature BMP-10 which forms disulfide-linked non-glycosylated homodimers (4, 5). In transfectants, BMP-10 is secreted as a cleaved mature dimer, an uncleaved proform dimer, and an uncleaved proform monomer (4). The propeptide of human BMP-10 shares 82% amino acid sequence identity with mouse and rat proBMP-10 and 19% - 34% with the propeptides of human BMP-9, GDF-5, -6, and -7. BMP-10 is critical for the proper development of the heart and first appears at the onset of trabeculation and chamber formation (6 - 8). Homozygous BMP-10 knockout mice die in utero due to arrested cardiac development (7). BMP-10 is required for maintaining expression of the cardiogenic transcription factors NKX2.5 and MEF2C in developing myocardium and promoting the growth of embryonic cardiomyocytes (7, 9, 10). The BMP-10 mediated proliferation of these cells requires Notch signaling (11). NKX2.5 itself negatively regulates BMP-10 expression in cardiac myocytes (10). Multiple human congenital heart defects result from mutations in NKX2.5 and require BMP-10 expression (10). In mice, genetic knockout of ErbB leads to a similar phenotype but appears not to involve BMP-10, and knockout of the calcium channel subunit FKBP12 induces BMP-10 overexpression (7). BMP-10 in the postnatal heart promotes increased cardiomyocyte and heart size (8). BMP-10 has been shown to induce signaling through ALK-1, BMPR-IA, BMPR-IB, and BMPR-II in transfectants and non-cardiac cell lines (4, 5). A functional BMP-10 receptor in the heart has not yet been identified, although deletion of BMPR-IA or BMP-10 causes similar cardiac morphogenetic abnormalities (12).

References
  1. Chen, D. et al. (2004) Growth Factors 22:233. 
  2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251. 
  3. Schneider, M.D. et al. (2003) Cytokine Growth Factor Rev. 14:1. 
  4. Mazerbourg, S. et al. (2005) J. Biol. Chem. 280:32122. 
  5. David, L. et al. (2007) Blood 109:1953. 
  6. Neuhaus, H. et al. (1999) Mech. Dev. 80:181. 
  7. Chen, H. et al. (2004) Development 131:2219.
  8. Chen, H. et al. (2006) J. Biol. Chem. 281:27481.
  9. Srivastava, D. and E.N. Olson (2000) Nature 407:221.
  10. Pashmforoush, M. et al. (2004) Cell 117:373.
  11. Grego-Bessa, J. et al. (2007) Dev. Cell 12:415.
  12. Gaussin, V. et al. (2002) Proc. Natl. Acad. Sci. 99:2878.
Long Name
Bone Morphogenetic Protein 10
Entrez Gene IDs
27302 (Human); 12154 (Mouse)
Alternate Names
BMP10; BMP-10; bone morphogenetic protein 10; MGC126783

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