Recombinant Human BMP-3b/GDF-10 Protein Summary
Product Specifications
Gln369-Arg478, with an N-terminal Met
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1543-BP
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 50 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1543-BP/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 4mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: BMP-3b/GDF-10
BMP-3b, also known as GDF-10, belongs to the transforming growth factor beta (TGF-beta ) superfamily which includes the TGF-beta s, bone morphogenetic proteins (BMPs), growth differentiation factors (GDFs), Activins, Inhibins, Leftys, Nodal, Mullerian inhibitory substance (MIS) and the glial cell line-derived neurotrophic factors (GDNFs) (1). TGF-beta family members are synthesized and secreted as homodimeric or heterodimeric prepropeptides that are cleaved by proprotein convertases such as furin to generate the carboxy-terminal mature dimeric protein that contains the characteristic conserved cysteine residues involved in the formation of the cysteine knot domain. Within pro-BMP-3b, three dibasic cleavage sites have been identified. Mature BMP-3B is a disulfide-linked homodimer of the C-terminal 110 amino acids. Among TGF-beta family members, BMP-3b is most closely related to BMP-3, sharing 83% and 30% amino acid sequence identity in their mature and pro regions, respectively (2, 3). BMP-3b is highly conserved across animal species, the aa sequence of mature human BMP-3b is 98% identical with that of the mouse or rat proteins (2, 3). BMP-3b is expressed in developing skeletal structures of the craniofacial region and the vertebral column, as well as in the adult trachea, aorta, and most abundantly in the cerebellum and uterus (2 - 4). Interestingly, in the knock-out mice, no obvious abnormalities have been found in these tissues (4). The biological function of BMP-3b is yet unknown, however studies have implicated it in the differentiation of osteoblasts, augmenting BMP-2 activity (5, 6).
- Miyazono, K. et al. (2001) J. Cell Physiol. 187:265.
- Cunningham, N.S. et al. (1995) Growth Factors 12:99.
- Takao, M. et al. (1996) Biochem. Biophys. Res. Comm. 219:656.
- Zhao, R. et al. (1999) Dev. Biol. 212:68.
- Hino, J. et al. (1999) Biochem.Biophys. Res. Comm. 256:419.
- Kaihara, S. et al. (2003) Life Sci. 72:1683.
Citations for Recombinant Human BMP-3b/GDF-10 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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GDF10 is a negative regulator of vascular calcification
Authors: Platko, K;Gyulay, G;Lebeau, PF;MacDonald, ME;Lynn, EG;Byun, JH;Igdoura, SA;Holden, RM;Roubtsova, A;Seidah, NG;Krepinsky, JC;Austin, RC;
The Journal of biological chemistry
Species: Mouse
Sample Types: In Vivo, Whole Tissue
Applications: Bioassay, In vivo assay -
GDF10 blocks hepatic PPARγ activation to protect against diet-induced liver injury
Authors: K Platko, PF Lebeau, JH Byun, SV Poon, EA Day, ME MacDonald, N Holzapfel, A Mejia-Beni, KN Maclean, JC Krepinsky, RC Austin
Mol Metab, 2019-06-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
GDF10 is a signal for axonal sprouting and functional recovery after stroke.
Authors: Li S, Nie E, Yin Y, Benowitz L, Tung S, Vinters H, Bahjat F, Stenzel-Poore M, Kawaguchi R, Coppola G, Carmichael S
Nat Neurosci, 2015-10-26;18(12):1737-45.
Species: Human
Sample Types: Whole Cells
Applications: Differentiation, Differentiation -
Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
J. Biol. Chem., 2011-07-07;286(34):30034-46.
Species: Human, Mouse
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance
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