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Recombinant Human BMP-5 (CHO-expressed) Protein

Catalog #: 615-BMC
8 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
615-BMC-020/CF

With Carrier

Catalog # Availability Size / Price Qty
615-BMC-020
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Product Details
Citations (8)
FAQs
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human BMP-5 (CHO-expressed) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.2‑1.2 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human BMP-5 protein
Ala317-His454
Accession #
P22003
N-terminal Sequence
Analysis
Ala317
Predicted Molecular Mass
15.6 kDa
SDS-PAGE
18-23 kDa, reducing conditions

Product Datasheets

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615-BMC (with carrier)

Product Datasheet
COA
SDS

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615-BMC/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

615-BMC

Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

615-BMC/CF

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-5

Bone Morphogenetic Protein-5 (BMP-5) is one of at least 15 structurally and functionally related BMPs which are members of the transforming growth factor  beta (TGF-beta ) superfamily (1). BMP-5 is synthesized as a 454 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 20 kDa C-terminal mature protein (2). Mature BMP-5 contains seven conserved cysteine residues involved in formation of the cysteine knot and the single interchain disulfide bond. Biologically active BMP-5 is a disulfide-linked homodimer of the C-terminal mature protein. Mature human BMP-5 shares 96% aa sequence identity with mouse and rat BMP-5. Cellular responses to BMP-5 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors (1). BMP-5 is expressed by chondrocytes in proliferating and hypertrophic zones of bone growth plates (3). It contributes to limb development by promoting proliferation and differentiation of chondrocytes as well as apoptosis of undifferentiated mesoderm (3, 4). Genetic defects in BMP-5 which cause C-terminal truncation or loss of the proteolytic cleavage site result in multiple skeletal abnormalities, including the short ear phenotype in mice (5, 6). BMP-5 is also expressed by ovarian granulosa cells where it functions as an autocrine factor to promote GC proliferation and inhibit their production of progesterone (7). In the nervous system, BMP-5 promotes dendrite outgrowth and dopaminergic neuronal differentiation (8, 9). It is up-regulated in oral squamous carcinoma cells and induces the apoptosis of some myeloma cell lines (10, 11).

References
  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. 87:9843.
  3. Mailhot, G. et al. (2008) J. Cell. Physiol. 214:56.
  4. Zuzarte-Luis, V. et al. (2004) Dev. Biol. 272:39.
  5. King, J.A. et al. (1994) Dev. Biol. 166:112.
  6. Ho, A.M. et al. (2008) BMC Dev. Biol. 8:35.
  7. Pierre, A. et al. (2005) Biol. Reprod. 73:1102.
  8. Beck, H.N. et al. (2001) BMC Neurosci. 2:12.
  9. Brederlau, A. et al. (2002) Mol. Cell. Neurosci. 21:367.
  10. Jin, Y. et al. (2001) Oral Oncol. 37:225.
  11. Ro, T.B. et al. (2004) Oncogene 23:3024.
Long Name
Bone Morphogenetic Protein 5
Entrez Gene IDs
653 (Human); 12160 (Mouse)
Alternate Names
BMP5; BMP-5; bone morphogenetic protein 5; MGC34244

Citations for Recombinant Human BMP-5 (CHO-expressed) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. G9a Knockdown Suppresses Cancer Aggressiveness by Facilitating Smad Protein Phosphorylation through Increasing BMP5 Expression in Luminal A Type Breast Cancer
    Authors: Y Jin, S Park, SY Park, CY Lee, DY Eum, JW Shim, SH Choi, YJ Choi, SJ Park, K Heo
    International Journal of Molecular Sciences, 2022-01-06;23(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Genomic analysis of benign prostatic hyperplasia implicates cellular re-landscaping in disease pathogenesis
    Authors: LW Middleton, Z Shen, S Varma, AS Pollack, X Gong, S Zhu, C Zhu, JW Foley, S Vennam, RT Sweeney, K Tu, J Biscocho, O Eminaga, R Nolley, R Tibshirani, JD Brooks, RB West, JR Pollack
    JCI Insight, 2019-05-16;5(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. BMP-induced reprograming of the retina into RPE requires WNT signalling in the developing chick optic cup
    Authors: J Steinfeld, I Steinfeld, A Bausch, N Coronato, ML Hampel, H Depner, PG Layer, A Vogel-Höpk
    Biol Open, 2017-07-15;0(0):.
    Species: Chicken
    Sample Types: In Vivo
    Applications: In Vivo
  4. A gradient of Bmp7 specifies the tonotopic axis in the developing inner ear.
    Authors: Mann Z, Thiede B, Chang W, Shin J, May-Simera H, Lovett M, Corwin J, Kelley M
    Nat Commun, 2014-05-20;5(0):3839.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Ligand- and stage-dependent divergent functions of BMP signaling in the differentiation of embryonic skeletogenic progenitors in vitro.
    Authors: Lorda-Diez C, Montero J, Choe S, Garcia-Porrero J, Hurle J
    J Bone Miner Res, 2014-03-01;29(3):735-48.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Repulsive guidance molecule (RGM) family proteins exhibit differential binding kinetics for bone morphogenetic proteins (BMPs).
    Authors: Wu, Qifang, Sun, Chia Chi, Lin, Herbert, Babitt, Jodie L
    PLoS ONE, 2012-09-27;7(9):e46307.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  7. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011-07-07;286(34):30034-46.
    Species: Human, Mouse
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance

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